Flavonol multi-site glucosyltransferase CsUGT73A20 gene as well as coding protein and application thereof

A glucose-based, protein-encoding technology, applied in the directions of transferase, application, genetic engineering, etc., can solve the problems of no market product, difficult purification, and low natural content.

Inactive Publication Date: 2015-11-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still some flavonoid glycosides, such as kaempferol dioxyglucoside, which are difficult to purify due to their low natural content, so far there is no commercial product on the market

Method used

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  • Flavonol multi-site glucosyltransferase CsUGT73A20 gene as well as coding protein and application thereof
  • Flavonol multi-site glucosyltransferase CsUGT73A20 gene as well as coding protein and application thereof
  • Flavonol multi-site glucosyltransferase CsUGT73A20 gene as well as coding protein and application thereof

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Embodiment Construction

[0025] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the following will combine the embodiments of the present invention and the embodiments Attached picture , clearly and completely describe the technical solutions in the embodiments of the present invention, obviously, the described embodiments are part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0026] 1. Mat...

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Abstract

The invention discloses a flavonol multi-site glucosyltransferase CsUGT73A20 gene. The gene is separated out from fresh tea leaves; the nucleotide sequence of the gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the coding protein of the gene is as shown in SEQ ID NO. 2. In the invention, the CsUGT73A20 gene is firstly cloned and verified that the CsUGT73A20 gene has a function of forming multi-site flavonol monoglucoside and diglucoside; the invention also provides a recombinant plasmid, a transgenic engineering bacterium and recombinant protein containing the CsUGT73A20 gene; and the mass synthesis of the multi-site flavonol monoglucoside and diglucoside is achieved by a biological engineering method, so as to lay a foundation for further implementation of researches on flavonol glucoside biosynthesis regulation.

Description

technical field [0001] The invention relates to the technical field of molecular bioengineering, in particular to a flavonol multi-site glucosyltransferase CsUGT73A20 gene, its encoded protein and its application. Background technique [0002] Flavonoids in plants belong to a class of natural compounds with benzopyran ring structure. They are mostly combined with sugar groups at the 3-O, 5-O, 7-O, 3'-O, 4'-O and other polyhydroxyl sites of the benzopyran ring core to form flavonoid glycosides. The flavonoids in tea tree are mainly naringenin (N), kaempferenin (KC), kaempferol (K) and apigenin (A), such as figure 1 shown. [0003] Flavonoids and flavonoid glycosides are important components of tea polyphenols, which are not only quality components such as astringency in tea soup, but also important factors that constitute the color of tea soup. Tea tree flavonoids and their glycosides have a variety of physiological functions such as anti-oxidation, etc., and are currently...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/70C12N1/21C12R1/19
Inventor 高丽萍赵贤倩
Owner ANHUI AGRICULTURAL UNIVERSITY
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