FSHR (follicle stimulating hormone receptor) gene based molecular marker related to porcine reproduction traits as well as detection method and application of molecular marker

A technology of molecular markers and detection methods, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem of no FSHR gene, etc., and achieve the effect of easy detection

Inactive Publication Date: 2015-11-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

The research reports on the polymorphism of FSHR gene mainly focus on humans, cattle, sheep, and goat

Method used

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  • FSHR (follicle stimulating hormone receptor) gene based molecular marker related to porcine reproduction traits as well as detection method and application of molecular marker
  • FSHR (follicle stimulating hormone receptor) gene based molecular marker related to porcine reproduction traits as well as detection method and application of molecular marker
  • FSHR (follicle stimulating hormone receptor) gene based molecular marker related to porcine reproduction traits as well as detection method and application of molecular marker

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Embodiment 1

[0028] In 2013, the ear samples of 106 Xiaomeishan pigs, 42 Fengjing pigs and 70 Large White pigs were collected; at the same time, the ear samples of the above 106 Xiaomeishan sows from 2004 to 2012 were sorted out Production files, a total of 698 litter piglet records, mainly collect indicators such as farrowing year, parity, TNB, NBA and so on. Ear samples (about 0.15 g) were collected with ear size forceps and frozen at -20°C. The genomic DNA of the pig ear sample was extracted by conventional phenol-chloroform extraction method, the OD value of the genomic DNA was measured, the concentration was calculated, and the ddH 2 Dilute O to a final concentration of 100ng / μL, store the original solution at -20°C, and store the diluted solution at 4°C.

[0029] Step 1: Obtain the exon sequence of porcine FSHR gene.

[0030] According to the complete sequence of human FSHR gene published by GenBank (accession number: NG_008146) and pig FSHRmRNA sequence (accession number: NM_21438...

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Abstract

The invention discloses an FSHR (follicle stimulating hormone receptor) gene based molecular marker related to porcine reproduction traits as well as a detection method and an application of the molecular marker. The molecular marker is a porcine FSHR gene segment which contains a polymorphic locus, and the polymorphic locus is at the 1,166th site in a sequence shown in the SEQ ID NO.1 and has C/T base variation. The detection method comprises the following steps: a primer is designed according to the nucleotide sequence of the molecular marker; PCR (polymerase chain reaction) amplification is performed with genomic DNA of a to-be-detected pig as a template; the type of a polymorphic locus in a PCR amplification product is judged. The invention further discloses the application of the molecular marker in selection of the porcine reproduction traits. According to an FSHR gene mutation site, the FSHR gene based molecular marker related to the porcine reproduction traits as well as the detection method and the application of the molecular marker are provided, and foundation is provided for porcine molecular breeding and development of a physiological mechanism for porcine reproduction.

Description

technical field [0001] The invention relates to the field of animal molecular breeding, in particular to a FSHR gene-based molecular marker related to pig reproductive traits and a detection method and application thereof. Background technique [0002] Molecular marker-assisted selection is an important application of genetic engineering in modern livestock breeding. It uses various markers related to breeding traits instead of phenotype-based selection, and analyzes the genetic composition of individuals at the molecular level, so as to achieve gene selection. Type of direct selection can overcome the disadvantages of direct selection, improve the accuracy of selection and shorten the generation interval. [0003] The reproductive traits of pigs, such as total litter size and live piglet size, are important economic traits, which are directly related to the economic benefits of pig farming. [0004] Follicle-stimulating hormone (FSH) is a glycoprotein hormone secreted by b...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴井生陈超郭苹陈永霞
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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