A method for using a special source of bacteria synthesized by natural understory ectomycorrhizal
An ectomycorrhizal and special-purpose fungus technology, applied in the field of mycology, can solve the problems of short survival time of artificially cultured mycelium, affecting large-scale, commercial development, and inability to carry out artificial reproduction, so as to facilitate large-scale popularization and application. , the effect of expanding the number of inoculation sources and easy operation
Inactive Publication Date: 2017-12-08
陈宇航
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Problems solved by technology
Trying to bypass this bottleneck, the method of moving the symbiotic seedlings of delicious boletus into the forest can not be successful (Hall I. R. Lyon A. J. E. Wang Y., 1998)
[0006] The inoculum sources commonly used in mycorrhizal technology include artificially cultivated mycelium and fungal spores. Artificially cultivated mycelium survives for a short time in forest soil rich in microbial species and is difficult to inoculate successfully; fungal spores can be obtained in forest soil Long survival period and high inoculation rate, but due to difficulty in collection, high cost, and inability to carry out artificial propagation, which affects the development of scale and commercialization, it is currently mainly used for mycorrhizalization experiments in natural forests
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[0020] The use of a special bacterial source for ectomycorrhizal fungus inoculation introduced by the present invention includes cultivating sterile forestry seedlings under strict aseptic conditions, and controlling temperature, light, water and humidity during any period between the growth of fine roots and the first anniversary of the seedlings Under certain conditions, the fine roots of seedlings are artificially inoculated with mycelium or spores of mycorrhizal fungi artificially isolated, and cultivated under conditions suitable for mycorrhizal growth for more than 4 weeks to obtain mature mycorrhizae. The obtained mycorrhizae can be observed under the microscope as the mycosheaths on the fine roots and the dense, outwardly extending hyphae, and even a large number of mycorrhizae can be observed with the naked eye ( Figure 5 ).
[0021] Cut the mycorrhizae with a diameter of less than 2mm, clean the floating mud with sterile water in an ultrasonic washing machine; test ...
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The invention discloses a method for using a special fungus source synthesized by natural understory ectomycorrhizae, cultivating healthy forestry seedlings, and implementing the mycorrhizalization technology of the seedlings to obtain mature mycorrhizae of target fungi. Carefully select healthy mycorrhizae, cut them into appropriate lengths, and mix them with a mixture of vermiculite, peat soil, and sterile water in proportion to make a source of inoculation bacteria, which can be used in natural forests or for mycorrhizal expansion Seedlings. The invention can not only obtain a large number of fungus sources with long survival time under natural forests and high inoculation success rate through artificial propagation, but also make up for the multiplication problem of a small number of mycorrhizal fungi that are difficult to separate and purify.
Description
technical field [0001] The invention belongs to the field of mycology, and in particular relates to a method for using a special fungal source synthesized by natural understory ectomycorrhizae. Background technique [0002] According to domestic reports, ectomycorrhizal edible fungi account for 677 / 966 of the known edible fungus species, and most of them belong to human health food because they contain essential active substances for human beings. The huge contribution will definitely become the development direction of the edible fungus industry in the future. Including russula, matsutake, delicious boletus, pine milk mushroom, chicken fir, red beef liver, yellow beef liver, dried mushrooms, but not limited to these types, quite a few types are recognized as world-renowned healthy + delicious food. [0003] The technology of infecting forestry trees with mycorrhizal fungi and forming symbionts is called mycorrhizal technology. Mycorrhizal technology is undoubtedly the onl...
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IPC IPC(8): A01G1/04C12N1/14
CPCA01G18/10A01G18/20C12N1/14
Inventor 陈宇航陈鹏达周仲驹陈柳英
Owner 陈宇航