Multi-term respiratory tract pathogene detection kit and detection method thereof

Abstract

The invention provides a multi-term respiratory tract pathogene detection kit and a detection method thereof. The multi-term respiratory tract pathogene detection kit comprises a sample extraction liquid, sample pipes and multi-joint inspection reagent plates. Each multi-joint inspection reagent plate comprises a connecting plate and at least one single reagent plate. Each single reagent plate comprises a clamping plate and a reagent strip, wherein one end of the clamping plate is provided with a connecting portion, the connecting portion is detachably connected with the corresponding connecting plate, and the upper surface of the clamping plate is provided with a sample adding hole and a detection window. Each reagent strip comprises a PVC plate, a chromatography membrane, a sample pad, a combined pad and a water absorbing material. An antibody corresponding to at least one kind of fluorescently-labeled respiratory tract pathogene to be tested is fixed to each combined pad. Each chromatography membrane comprises a quality control line C and at least one detection line T, wherein an antibody corresponding to the corresponding respiratory tract pathogene to be tested is fixed to the line T, and a goat anti-mouse igG polyclonal antibody is fixed to the line C. The kit is easy, convenient and fast to operate, the detection time is short, the detection specificity is high, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to a multiple respiratory pathogen detection kit and a detection method thereof. Background technique [0002] The existing seven-item respiratory pathogen detection kit (immunofluorescence method) combines the fluorescein-labeled antibody with the virus antigen on the cell to form an antibody-antigen complex, and observes the number of cells showing green fluorescence under a fluorescent microscope. [0003] Main technical defects: (1) The sample processing process is complicated, and the sample needs to be centrifuged and washed several times to remove the mucus layer to make a cell suspension; (2) The detection process is complicated and takes 1-2 hours. (3) Fluorescence microscopes are required, the instruments are expensive, and technicians are required to operate. (4) Qualitatively detect the presence or absence of virus in the sample, but the number of viruses cannot be determ...

AI Technical Summary

Problems solved by technology

[0003] Main technical defects: (1) The sample processing process is complicated, and the sample needs to be centrifuged and washed several times to remove the mucus layer to make a cell suspension; (2) The detection process is complicated and takes 1-2 hours for a long time

(3) Need to use fluorescence microscope, the instrument is expensive, and requires technicians to operate

(4) Qualitatively detect the presence or absence of virus in the sample, but the number of viruses cannot be determined

(5) Only viruses that infect cells can be detected, and free viruses in samples cannot be detected; when a small number of viruses infect cells, it may be due to the small number of infected viruses that green fluorescent cells cannot be observed, resulting in false negatives

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