A cold-water fish probiotic Bacillus strain and its application

A technology of bacillus and cold water fish, applied in the field of microorganisms, can solve the problems of low antibody titer and antibody titer, etc.

Inactive Publication Date: 2019-09-03
哈尔滨国生生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish have the ability of immune memory response. If you want to obtain a strong immune response again, it will take a long time, and the antibody titer produced by the secondary response is lower than that of mammals.

Method used

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  • A cold-water fish probiotic Bacillus strain and its application
  • A cold-water fish probiotic Bacillus strain and its application
  • A cold-water fish probiotic Bacillus strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Bacillus of the present invention HZC58 Separation, purification and identification of

[0032] GM17 solid medium formula (g / L):

[0033] Soytone 5.0, peptone 2.5, casein peptone 2.5, yeast extract powder 2.5, beef powder 5.0, lactose 5.0, sodium ascorbate 0.5, β-sodium glycerophosphate 19.0, magnesium sulfate 0.25, agar 15, after dissolving, sterilize at 110°C for 15 minutes, Adjust the pH to 7.2 and pour the plate for later use.

[0034] GM17 liquid medium formula (g / L):

[0035] Soytone 5.0, peptone 2.5, casein peptone 2.5, yeast extract powder 2.5, beef powder 5.0, lactose 5.0, sodium ascorbate 0.5, β-sodium glycerophosphate 19.0, magnesium sulfate 0.25, after dissolution, sterilize at 110°C for 15 minutes, adjust pH 7. 2, spare.

[0036] (1) Isolation and purification of strains:

[0037] Juvenile rainbow trout from the semi-wild mountainous area of ​​Benxi, Liaoning Province should minimize the stress of rainbow trout, quickly disinfect the body ...

Embodiment 2

[0043] Embodiment 2 Bacillus of the present invention HZC58 A study on the acid-resistance characteristics of strains

[0044] The Bacillus HZC58 Unique acid-resistant properties of the strain:

[0045] (1) Experimental method: the Bacillus HZC58 The strains were cultured at 30°C, and the growth of the strains in GM17 liquid medium with different pH (pH3.0, pH4.0, pH5.0, pH6.0 and pH6.5) was observed.

[0046] (2) Experimental results: the Bacillus HZC58 The strain has good adaptability in medium with different pH. It can proliferate in the acidic GM17 medium with a pH of 4.0; it can proliferate faster in the slightly acidic GM17 medium with a pH of 5.0; the near-neutral pH of 6.5 Proliferate rapidly in GM17 medium. See figure 2 Proliferation curve, indicating that the Bacillus HZC58 Possesses the ability to tolerate the low pH environment of the intestinal tract, indicating that Bacillus HZC58 The strain has the ability to colonize in the acidic environment of the in...

Embodiment 3

[0047] Embodiment 3 Bacillus of the present invention HZC58 Study on the characteristics of cholate resistance of the strain

[0048] The Bacillus HZC58 Unique cholate-tolerant properties of the strain:

[0049] (1) Experimental method: the Bacillus HZC58 The strains were cultured at 30°C, and the growth of the strains in GM17 liquid medium containing different cholate concentrations (cholate concentrations of 0.10%, 0.20%, 0.30%, 0.40% and 0.50%) was observed.

[0050] (2) Experimental results: Rapid proliferation can be achieved in GM17 medium containing 0.0%-0.2% cholate; rapid proliferation can still be achieved in GM17 medium containing 0.3-0.5% cholate. See image 3 Proliferation curves, indicating that Bacillus HZC58 The strain has the ability to colonize intestinal hypercholate.

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Abstract

The invention discloses a cold-water-fish probiotics bacillus strain and application thereof. The strain HZC58, which has anti-virus, immune adjustment and immunity enhancement characteristics for cold water fishes, is separated and identified from an intestinal tract mucous membrane of a juvenile semi-wild rainbow trout in Benxi mountainous area of Liaoning province. According to colony morphological characteristic observation, 16SrDNA base sequence determination and homology analysis, the identification result of the strain indicates that the HZC58 belongs to bacillus and the collection number is CGMCC No.10706. In-vitro cell experiments prove that the bacillus strain HZC58 is cultured together with EPCs (endothelial progenitor cells) and has the characteristic of resisting IHNV (infectious hematopoietic necrosis virus) in EPC proliferation in vitro. Animal experiments prove that the bacillus strain HZC58 has the immune adjustment and immunity enhancement characteristics for the cold water fishes, and the strain HZC58 has also the anti-virus characteristic for the cold water fishes when being used for feeding individually. The bacillus strain HZC58 has a certain application prospect in cold-water-fish anti-virus, immunity adjustment and immunity enhancement.

Description

technical field [0001] The invention relates to a strain of probiotic bacteria and its application, in particular to a strain of cold-water fish (rainbow trout) probiotic bacillus HZC58 The bacterial strain and its application belong to the technical field of microorganisms. Background technique [0002] Probiotics are the normal flora of human and animal intestinal tract, respiratory tract, reproductive tract and skin mucous membrane system. Many research results have found that lactic acid bacteria and intestinal mucosa together form a protective barrier to prevent the invasion of viruses and bacteria. Human beings are increasingly aware of In view of the powerful effect of probiotics on human and animal life and health, a large number of modern immunological research results have confirmed that probiotics (especially lactic acid bacteria) can regulate animal cellular immunity, humoral immunity and local immunity of intestinal mucosa, as well as immune system development a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/80A23K20/195A61K39/39A61K39/205A61K35/742A61K39/385A61P31/14A61P37/02A61P37/04C12R1/07
CPCY02A40/818
Inventor 王云峰崔红玉王笑梅
Owner 哈尔滨国生生物科技股份有限公司
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