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Improved erythrocyte osmotic fragility detection method

A technology of osmotic fragility and detection method, applied in the field of blood disease detection, can solve the problems of polluting water sources and adverse health effects of nearby residents

Inactive Publication Date: 2015-12-09
合肥金域医学检验实验室有限公司
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Problems solved by technology

If the sewage after large-scale testing in the laboratory is not properly treated, it will pollute the water source and cause adverse effects on the health of nearby residents

Method used

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  • Improved erythrocyte osmotic fragility detection method
  • Improved erythrocyte osmotic fragility detection method
  • Improved erythrocyte osmotic fragility detection method

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Experimental program
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Embodiment Construction

[0019] 1. Materials:

[0020] 1.1 Objects: Randomly select 100 cases of erythrocyte osmotic fragility test samples submitted to our center in October 2014, 2.0ml of EDTA-K2 anticoagulant blood, and conduct comparison and verification statistical analysis of various test methods.

[0021] 1.2 Instruments: RT-9600 semi-automatic biochemical analyzer, TECANSUNRISE microplate reader, 37°C constant temperature water bath

[0022] 1.3 Reagents: A mother liquor is sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate aqueous solution; B mother liquor is sodium chloride aqueous solution, and C mother liquor is double distilled water (D.H 2 O) or saponin solution.

[0023] 1.4 Judgment standard: The acceptable standard ALE of the results of re-examination of the reserved samples for this project is ±20%;

[0024] 1.5 Reference range: the adult reference value range is hemolysis% 65-100% (negative),

[0025] 55-65% is the critical range

[0026] The reference val...

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Abstract

The invention provides an improved erythrocyte osmotic fragility detection method. A microplate reader is employed to replace a semi-automatic biochemical analyzer or a spectrophotometer to detect hemolysis situations, and double distilled water replaces a saponin solution and is employed as a C mother solution in the detection method. In addition, the invention also provides an improved erythrocyte osmotic fragility detection kit. The detection kit is composed of three mother solutions A, B and C. The A solution is an aqueous solution of sodium chloride, disodium hydrogen phosphate and sodium dihydrogen phosphate. The B mother solution is an aqueous solution of sodium chloride. The C mother solution is double distilled water (D.H20).

Description

technical field [0001] The invention belongs to the field of blood disease detection. More specifically, the invention provides an improved erythrocyte osmotic fragility detection method, using a microplate reader instead of a semi-automatic biochemical analyzer or a spectrophotometer to detect hemolysis, and using double distilled water instead of soap The prime solution is used as the C mother solution in the detection method. In addition, the present invention also provides an improved erythrocyte osmotic fragility detection kit, which is composed of three mother solutions A, B and C, wherein A mother solution is sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate aqueous solution, B mother solution It is an aqueous solution of sodium chloride, and the mother liquor of C is double distilled water. Background technique [0002] Thalassemia is also called Thalassemia. Is a group of hereditary hemolytic anemia. Their common feature is that due to the...

Claims

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Application Information

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IPC IPC(8): G01N21/25
Inventor 张玲
Owner 合肥金域医学检验实验室有限公司
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