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A method for detecting organophosphorus pesticides in textiles

A technology for organophosphorus pesticides and textiles, applied in the field of detection, can solve the problems of expensive instruments, complicated operation procedures, cumbersome sample pretreatment, etc., and achieve the effects of low instrument prices, improved detection efficiency, and short test time.

Inactive Publication Date: 2018-03-20
JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These instrumental analysis methods have the characteristics of simple operation, fast analysis speed, good separation efficiency, high sensitivity, and wide application range, but they have high technical content, cumbersome sample pretreatment, relatively complicated operating procedures, expensive instruments, and require specific experiments. Conditions and professionals to operate, not suitable for on-site rapid analysis

Method used

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  • A method for detecting organophosphorus pesticides in textiles
  • A method for detecting organophosphorus pesticides in textiles
  • A method for detecting organophosphorus pesticides in textiles

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0030] The first step is to draw the standard curve, including the following steps:

[0031] (1) Mix 2nmol / L gold glue, 0.2nmol / L 4-aminophenyl phosphate, 2mmol / L silver nitrate and 0.04μg / mL alkaline phosphatase to obtain a mixed solution;

[0032] (2) Use a spectrophotometer to measure the absorbance of the mixed solution at 365 nm;

[0033] (3) Divide the mixture into 5 parts, add the standard solutions of methamidophos at concentrations of 0.05ng / ml, 0.5ng / ml, 5ng / ml, 50ng / ml and 500ng / ml respectively, mix them and leave them for 0.5h. Measure the absorbance value at 365nm; use the logarithm of the concentration values ​​of different methamidophos standard solutions as the abscissa and the absorbance value as the ordinate to draw a standard curve, such as figure 1 Shown.

[0034] The second step, sample pretreatment: first cut the textile sample into 5mm×5mm pieces, weigh 5g, add 20mL 0.2mmol / L phosphate buffer, and ultrasonically extract at 90°C for 30 minutes. The liquid is the...

Embodiment 2

[0042] The first step is to draw the standard curve, including the following steps:

[0043] (1) Mix 2nmol / L gold glue, 0.2nmol / L 4-aminophenyl phosphate, 2mmol / L silver nitrate and 0.04μg / mL alkaline phosphatase to obtain a mixed solution;

[0044] (2) Use a spectrophotometer to measure the absorbance of the mixed solution at 365 nm;

[0045] (3) Divide the mixture into 5 parts, add dimethoate standard solutions with concentrations of 0.05 ng / ml, 0.5 ng / ml, 5 ng / ml, 50 ng / ml and 500 ng / ml respectively, mix well and place for 5 hours to determine The absorbance value at 365nm; the logarithm of the concentration value of dimethoate standard solutions of different concentrations is used as the abscissa, and the absorbance value is used as the ordinate to draw a standard curve, such as figure 2 Shown.

[0046] The second step, sample pretreatment: first cut the textile sample into 5mm×5mm pieces, weigh 5g, add 20mL 0.2mmol / L phosphate buffer, and ultrasonically extract at 90°C for 30 mi...

Embodiment 3

[0054] The first step is to draw the standard curve, including the following steps:

[0055] (1) Mix 2nmol / L gold glue, 0.2nmol / L 4-aminophenyl phosphate, 2mmol / L silver nitrate and 0.04μg / mL alkaline phosphatase to obtain a mixed solution;

[0056] (2) Use a spectrophotometer to measure the absorbance of the mixed solution at 365 nm;

[0057] (3) Divide the mixed solution into 5 parts, add monocrotophos standard solutions with concentrations of 0.05ng / ml, 0.5ng / ml, 5ng / ml, 50ng / ml and 500ng / ml respectively, mix them and place them for 10h to determine Its absorbance value at 365nm; taking the logarithm of the concentration of monocrotophos standard solutions of different concentrations as the abscissa, and taking the absorbance value as the ordinate, draw a standard curve, such as image 3 Shown.

[0058] The second step, sample pretreatment: first cut the textile sample into 5mm×5mm pieces, weigh 5g, add 20mL 0.2mmol / L phosphate buffer, and ultrasonically extract at 90°C for 30 min...

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Abstract

The invention discloses a method for detecting organic a phosphorus pesticide in textiles, and relates to the detection field. The method comprises firstly drafting a standard curve, then shearing a sample into fragments, adding a buffer into the fragments and uniformly oscillating, so as to obtain a solution which is an extraction solution; successively adding alkaline phosphatase, silver nitrate, colloidal gold nanoparticle and a 4-aminophenylphosphate into the extraction solution for reaction, so as to obtain a reaction solution after the reaction is finished; and finally determining the absorbance value of the reaction solution at 365 nm by using an UV spectrophotometer, substituting the absorbance value into the equation of the standard curve, so as to obtain the organic phosphorus pesticide concentration in the textiles through calculation. The method is capable of accurately detecting the organic phosphorus pesticide concentration. Also, existence of the organic phosphorus pesticide can be visually determined through color variation, and thus the method can be used as a detection screening method for the organic phosphorus pesticides.

Description

Technical field [0001] The invention relates to the field of detection, in particular to a method for detecting organophosphorus pesticides in textiles. Background technique [0002] In the planting process of natural fiber crops (such as cotton, flax, etc.), they are susceptible to various pests and diseases and weeds, and pesticides need to be used regularly to ensure the normal growth of crops. At the same time, pesticides are also used to control parasites on livestock. Therefore, whether it is natural cellulose fibers or natural protein fiber textile materials, there may be residual pesticides. Although the final residues of pesticides in textile fiber raw materials may be quite limited, they may still pose a threat to human health and cause pollution to the environment. At present, the internationally renowned eco-labels Oeko-Tex Standard 100 and Eco-label both restrict a variety of pesticides in textiles. [0003] The conventional detection methods of pesticides include a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
Inventor 吴丽娜吴梦笔王亚春何重辉曹锡忠蔡建和
Owner JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE