Health reconciled milk beverage with nattokinase and preparation method thereof

A milk drink and soybean kinase technology, which is applied in the field of food and microbial fermentation engineering, can solve the problems of low popularity rate, not suitable for taste, complex preparation conditions, etc.

Inactive Publication Date: 2015-12-16
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nattokinase products currently on the market are mainly based on microbial solid-state fermented natto. The natto produced in this way has an ammonia smell, which is not suitable for the taste of Chinese people. The preparation conditions are relatively complicated and the popularity rate is not high.
However, there is no report on milk drinks containing nattokinase

Method used

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  • Health reconciled milk beverage with nattokinase and preparation method thereof
  • Health reconciled milk beverage with nattokinase and preparation method thereof
  • Health reconciled milk beverage with nattokinase and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A health-care blended milk drink containing nattokinase in this embodiment is prepared from soybean milk, milk and natto Bacillus subtilis seed liquid.

[0061] The preparation method of a kind of health-care blended milk drink containing nattokinase of the present embodiment is:

[0062] Add 50g of soybeans to 450mL of water to make soybean milk, filter 3 times, and sterilize the filtrate at 115°C for 30min, then set aside. Dissolve 45g of milk powder into 300mL and sterilize at 65°C for 30min. After the above two kinds of liquids were uniformly mixed at a volume ratio of 1:1, 3% of the total volume of the mixed liquid was added to the logarithmic phase of Bacillus subtilis natto seed liquid, and the shake flask was fermented at 160rpm and 35°C for 12h.

Embodiment 2

[0064] The difference between this example and Example 1 lies in that: the volume ratio of soybean milk and milk is 1:2; during fermentation, the shake bottle fermentation is carried out at 180 rpm and 40° C. for 8 hours.

Embodiment 3

[0066] A health-care blended milk drink containing nattokinase in this embodiment is prepared from soybean milk, milk, essence and Bacillus subtilis natto seed liquid.

[0067] The preparation method of a kind of health-care blended milk drink containing nattokinase of the present embodiment is:

[0068] Add 50g of soybeans to 450mL of water to make soybean milk, filter 3 times, and sterilize the filtrate at 115°C for 30min, then set aside. Dissolve 45g of milk powder into 300mL and sterilize at 65°C for 30min. After the above-mentioned two kinds of liquids are uniformly mixed with a volume ratio of 1:2, the logarithmic phase Bacillus subtilis natto seed liquid with a total volume of 3% of the mixed solution is added, and sweet-scented osmanthus essence with a total volume of 1% of the mixed solution is added, 160rpm, 35 ℃ shake flask fermentation for 12h.

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Abstract

The invention provides a health reconciled milk beverage with nattokinase. The health reconciled milk beverage is prepared by soybean milk, milk and bacillus subtilis natto seed liquid. The invention also provides a preparation method of the health reconciled milk beverage. The preparation method comprises the following steps: grafting the bacillus subtilis natto seed liquid in the soybean milk and the milk and fermenting. The health reconciled milk beverage prepared according to the invention has fibrinolytic activity, wherein the nattokinase activity is 160-185 U/mL; the health reconciled milk beverage is free of ammonia odor, relatively good in flavor and relatively high in stability, so that the health reconciled milk beverage can be stably stored for 3-5 days. The reconciled milk beverage prepared according to the invention has certain functions of fibrinolysis and health-care and is suitable for Chinese taste.

Description

technical field [0001] The invention belongs to the technical field of food and microbial fermentation engineering, and in particular relates to a health-care blended milk drink containing nattokinase and a preparation method thereof. Background technique [0002] Milk drinks have become an indispensable daily drink for Chinese people. Facing different needs, the types of milk beverages are increasing day by day. At present, domestic milk drinks mainly use soy milk, milk, or blended milk with the above as the main raw materials, such as fruit juice, fruit and vegetable multivitamins, vegetable protein, etc., and its main function is still to provide nutrition. In order to target specific health audiences, some special milk drinks with nutrition and health functions have emerged as the times require, such as low-fat milk, peanut milk, five-grain milk, chrysanthemum milk and black sesame milk. [0003] The World Health Organization (WHO) survey shows that the number of peopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/12
Inventor 聂光军岳文瑾赵锐聂智杰张枭董阿强朱香香朱竹兵刘芳
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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