191 results about "Bacillus subtilis (natto)" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a fermented liquid feed for pig and poultry as well as a preparation method and an application thereof, belonging to the field of biotechnology, in particular to a liquid feed produced by the fermentation of a plurality of beneficial microbes and Chinese medicinal herbs as well as a preparation method and an application thereof. The preparation method has simple process and comprises the following steps: after primary slant culture and secondary solution culture, lactobacillus, fermentation lactobacillus, bacillus subtilis, natto bacillus and saccharomyces cerevisiae are put into a solid fermenting tank with a fermentation medium containing astragalus root, Na2HPO4, nitrogen source and carbon according to weight proportion. The fermented liquid feed adopting the method has high quality, thicker mellow flavor and short fermenting time, contains protein, polypeptide, small peptide, amino acid, astragalus polyose and a plurality of beneficial microbes and is applicable to preparing various perfect compound feeds for pig and poultry.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention discloses a composite bacillus preparation applied to improving the aquatic environment of mariculture, and a making method thereof. The preparation is made by mixing Bacillus subtilis, Bacillus licheniformis, Bacillus cereus and Bafillus natto according to certain ratio, and under room temperature, the active microorganism is in resting state and the preparation is stable and easy to be preserved. After the composite bacillus preparation is put into mariculture water, the dormant bacteria can be back to normal quickly, degrade residual bait, dejection, fertilizer, biological residual bodies and other organic contaminant fast, reduce the content of ammonia nitrogen, nitrite and COD in the culture water and reproduce to be predominant bacteria in the water quickly so as to further restrain the putrefying bacteria from reproduction, improve the environment of the culture water and purify the water. The preparation of the invention is safe, contains no pollution nuisance and residual, can obviously improve the environment of the culture water, and meanwhile, the immunity of aquatic livestock can be improved.

The invention relates to a simple and efficient fermented soybean meal production technology and an application thereof. The preparation method comprises the following steps of: mixing soybean meal with a Chinese herbal medicine mixture of liquorice, wild chrysanthemum, folium ginkgo and pericarpium citri reticulatae viride at a ratio of 100:(1-2), adding composite zymocyte consisting of succinic acid generating bacillus filiformis, bacteroides ruminicola, bacillus subtilis natto and lactic acid bacteria, and performing mixed anaerobic fermentation. The release and absorption of active substances of the Chinese herbal medicine can be promoted, and the fermentation efficiency of the fermented soybean meal can be improved. Through low-temperature drying, the fermented soybean meal can be used for completely or partially replacing fish meal and for completely or partially replacing antibiotics in pig feed production, and can effectively improve the food intake of live pigs and the utilization rate of feed, promote the growth of live pigs, improve the immunity, enhance the disease resistance and improve the pork quality.

The invention discloses a natto beverage and a preparation method thereof. The natto beverage contains a fermentation product obtained by deep fermenting beans with natto bacillus liquid, the used natto bacillus is a bacillus subtilis natto subspecies ODK-BN1 strain with the preservation number of CCTCC No. M207055; the preparation method of the natto beverage comprises the steps of: firstly deep fermenting beans with natto bacillus liquid to obtain a fermentation product, and then preparing the liquid fermentation product and edible auxiliaries into beverages according to a conventional method. By adopting the natto bacillus strain which is anoxia-tolerant, high-temperature resistant and has high yield of nattokinase, and adopting a liquid deep fermentation method to prepare the natto beverage, therefore, the invention has the advantages of simple method, low cost, a plurality of beneficial physiologically-active components in the product, attractive appearance and flavour, safe and non-toxic, good stability, long preservation time, easy acceptance by consumers and broad market prospects.

The invention relates to a barton fermentation bed complex microbial inoculant and a preparation method thereof. The invention is characterized in that the complex microbial inoculant comprises the following six beneficial microorganisms in percentage by weight: 15-30 percent of Trichoderma koningii, 10-25 percent of geotrichum candidum, 10-25 percent of Candida utilis, 10-30 percent of lactobacillus plantarum, 10-25 percent of bacillus subtilis and 5-15 percent of bacillus subtilis natto. The complex microbial inoculant is prepared by a fermentation process, and the total number of fermentation viable bacteria in finished products is 2-5*10<9>cfu/ml. The microbial strains adopted by the complex microbial inoculant are safe and reliable, the finished viable bacteria content is high and the bioactivity is high; 1L/m<3> complex microbial inoculant is used on a barton fermentation bed, thus obviously reducing the odor in a barton, decreasing the breeding of mosquitoes and flies, having stronger degradation capability on chicken excrement, achieving the ecologically breeding purposes of no pollution and zero emission, enhancing the digestive absorption function of chicken, improving the chicken immunity, enhancing the broiler quality, increasing the quality of eggs, and ensuring the color of yolks to be brightly red.

The invention relates to a bacillus subtilis strain for excreting nattokinase at high efficiency. An aprN gene of bacillus subtilis NK is amplified by a PCR (polymerase chain reaction) technique; codons of initial thirty amino acids are optimized according to the codon preference of the bacillus subtilis, a recombination expression plasmid pHT01-aprN is established, and the correctness is verified through the restriction enzymes digestion, PCR amplification and sequencing. The recombination expression plasmid pHT01-aprN is imported into the bacillus subtilis 168 by an electric shocking method, and is performed with resistance screening by adopting chloramphenicol, so as to obtain engineering bacteria. After the fermenting condition is optimized, and the expression is induced by IPTG, the highest enzyme activity in a shake culture fermenting liquid is 289U/ml; the highest enzyme activity in a high-density fermenting liquid is 7778U/ml; the highest enzyme activity of a nattokinase preparation prepared by affinity column chromatography is 981731U/g.

The present invention provides Natto manufactured by mixing steamed soybean, cooked barley and yam, inoculating Bacillus subtilis Natto to the mixture, and fermenting the mixture, which is almost odorless, soft, good taste, and viscous, and further, functional substances such as vitamin K2 and enzyme (Nattokinase and Protease) are remarkably increased. This product may extend the utilization as functional traditional foods relating to prevention of life-style related disease.

The invention provides bacillus subtilis natto ST188 with CGMCC No. 8400 and a high-yield method for purifying vitamin menadione-7 by using the bacterial strain. The high-yield method comprises the following steps: (1) carrying out spray drying on natto fermentation liquor, and extracting or leaching bacillus natto fermentation liquor spray drying powder by using a solvent so as to obtain a leaching solution; (2) concentrating the leaching solution obtained in the step (1) so as to obtain extract; (3) carrying out column chromatography on the extract obtained in the step (2), carrying out gradient or isocratic elution, and concentrating collected liquid, thus obtaining a menadione-7 crude product; and (4) crystallizing and purifying the menadione-7 crude product obtained in the step (3), thus obtaining the pure vitamin menadione-7 product.

The invention relates to the field of microorganisms, and specifically relates to bacillus subtilis LSSE-22 and an application thereof. The invention provides the bacillus subtilis LSSE-22 with the preservation number of CGMCC No.4970. The invention also provides the application of the bacillus subtilis LSSE-22. The invention provides a natto food which is prepared by fermenting bean substrates through the bacillus subtilis LSSE-22. The bacillus subtilis LSSE-22 has higher natto kinase production activity, and when chick-peas are used as substrates, the highest activity of natto kinase reaches 356.25FU/g (the dry weight of the chick-peas). The natto kinase and an antioxidant active substance total phenol are prepared through simultaneous separation by using an ethanol separation method, the activity of the natto kinase reaches 2852.62FU/g (dry weight), the content of the total phenol reaches 14.78mg gallic acid/g (dry weight), and the method is simple and efficient.

Provided is a production method for bacillus natto and cordyceps fungus composite enzyme series of products. The main products comprise bacillus natto and cordyceps fungus composite low molecular weight enzyme beverage, composite enzyme capsules and composite enzyme tablets. The production method comprises a single-microorganism fermentation technology and a mixed-microorganism fermentation technology. The bacillus natto employs bacillus subtilis natto, and the cordyceps fungus employs cordyceps sinensis or cordyceps militaris. The bacillus natto can inhibit pathogenic bacteria. The generated nattokinase can dissolve thrombus, inhibit generation of angiotensin and reduce blood pressure. No acids are generated during fermentation of the bacillus natto, and no sweeteners need to be added into the products, which is beneficial to health. The cordyceps fungus has immune modulating functions, has anti-tumor, bacteriostasis and antiinflammation effects, and has some effects on nervous systems and livers. The effective substances of the products are stable active ingredients generated from the living microorganism, and with low molecular weight, such as peptides with low molecular weight, oligosaccharides, saponins, isoflavone, cordycepin, phosphatidylcholine, trace elements and the like. The products do not need to be stored at low temperature and has a long storage life. The products contain essential amino acids and vitamins of human body, and are nutritive and healthy.

The invention discloses bacillus subtilis natto for high yield rate of vitamin K2 (MK-7) and application thereof, and belongs to the field of microorganisms. The bacillus subtilis natto ND-1-A27 was collected into China Center for Type Culture Collection on March 14, 2018 in Wuhan University of China, and the collection number is CCTCC NO:M 2018131. The bacillus subtilis natto has the advantages that the yield rate of the vitamin K2 (MK-7) is greatly increased and is about 70mg/L, and the maximum yield rate can be reached by about three days; the fermenting cycle is greatly shortened, the timeis shortened, and the cost is reduced; the bacillus subtilis natto can be applied to industrialized fermenting production, and the genetic stability is good; after five generations are continuously transferred, the yield rate of the vitamin K2 (MK-7) is basically stable, and is maintained at the higher level; the bacillus subtilis natto can be used as a production bacterial strain for further study and development.

The invention relates to microecological preparations, and specifically relates to a microecological preparation produced through composite bacillus spp. solid state fermentation, and a preparation method thereof. The composite bacillus spp. microecological preparation provided by the invention is prepared from any two or more than two of bacillus subtilis, blichenif irmis, B.natto, B.licoglans, B.lentus and B.pumilus. The materials are mixed, and are processed through solid state fermentation, such that a composite preparation is obtained. With the composite bacillus spp. microecological preparation provided by the invention, pollutants such as organics, nitrogen and phosphorus in a cultivation pond can be effectively decomposed, and a water body environment of intensive aquaculture can be repaired. According to the invention, the microecological preparation is produced from composite bacillus spp., which is prepared with cheap culture mediums and the solid state fermentation technology. Therefore, the preparation and the method provided by the invention assist in the popularization and application of microbial preparations.

The invention provides a beneficial fungus feed additive for large-scale composite edible fungi and a method for breeding flavored pigs. The beneficial fungus feed additive is formed by compounding a beneficial fungus mixture and the large-scale edible fungi, wherein the beneficial fungus mixture consists of lactobacillus, streptococcus faecalis, saccharomycetes, bacillus subtilis, bacillus subtilis natto and bacillus licheniformis, and live bacterial counts of the beneficial fungus mixture range from 0.8 to 1 billion/gram; the beneficial fungus mixture and the crushed fresh large-scale edible fungi are mixed in a mass ratio of 1:(0.8-1.2), and are placing at the temperature of between 33 and 37 DEG C for 3 to 5 days to obtain the beneficial fungus feed additive for the large-scale composite edible fungi. The method for breeding he flavored pigs comprises the following steps of: adding the beneficial fungus feed additive for the composite edible fungi into feed in a ratio of 0.1 to 0.5 mass percent feeding the pigs before slaughter for 30 to 60 days and thus, obtaining green flavored pork products.

The invention provides a method for producing gamma-polyglutamic acid through adding sodium chloride (NaCl) in a fermentation process. According to the method, bacillus subtilis natto is used as a producing bacterium for the gamma-polyglutamic acid. The method comprises the following main three fermentation processing steps: performing aerobic culture on the producing bacterium in a liquid fermentation medium for a period of time in advance; then, adding the NaCl so as to increase the osmotic pressure of a fermentation substrate; and then, continuing performing the aerobic culture to a fermentation end and the like. Compared with the yield of the gamma-polyglutamic acid produced through adding the NaCl before inoculation or not adding the NaCl, the yield of the gamma-polyglutamic acid produced by the method for producing gamma-polyglutamic acid through adding the NaCl in the fermentation process can be obviously increased. Moreover, the method for producing gamma-polyglutamic acid through adding the NaCl in the fermentation process is simple in operation, is convenient for large-scale production and has a good application prospect.

The invention discloses a microbiological preparation for efficiently treating municipal lake water pollution. The microbiological preparation is prepared from nitrosospira, rhodospirillum, bacillus subtilis natto, kitasatospora phosalacinea and lactobcillus in a volume ratio of 0-10 to 5-15 to 5-15 to 5-15 to 5-15. The microorganism selected and cultured by the invention has the capability of being rapidly multiplied and developed into a dominant microflora under the municipal lake water environment; and each strain can independently and cooperatively decompose eutrophic ingredients in the municipal lake water and can make certain eutrophic ingredients which are difficult to decompose in a normal state easy to decompose. The microbiological preparation has the advantages of purifying the water quality well, improving the substrate, optimizing the biological environment, saving the biological environment from damage, having obvious effect on water raising, water retention, water color stabilization, treatment of turbid water, water concentrate, red water, black water, bottom odourization and algae aging and regulation and stabilization of pH value of the water body and making the lake water clear and odorless rapidly, along with simple using method, low cost, obvious effect, no secondary pollution.

The invention discloses a bio-additive premixed feed special for pigs and a method for preparing a bioactive substance carrier thereof. The bio-additive premixed feed special for the pigs contains the bioactive substance carrier, calcium dihydrogen phosphate, calcium carbonate, a vitamin additive, an amino acid additive, mineral elements, an enzyme preparation, a mycotoxin adsorbent, lactose, a liquid bioactive substance of a bacillus subtilis natto subspecies Chou 007 of which the dry matter quantity is between 10 and 40 percent, and a liquid bioactive substance of a lactobacillus sporogenes 90CT172 of which the dry matter quantity is between 10 and 40 percent, wherein the bioactive substance carrier is prepared from the bioactive substances of the bacillus subtilis natto subspecies Chou 007 and the lactobacillus sporogenes 90CT172 and a solid state fermentation bioactive substance which are added with seaweed meal. The bio-additive premixed feed special for the pigs prepared by the technique has low cost, is a green feed for feeding the pigs, and is a novel feed without side effects, residues, drug resistance or toxicity.

The invention discloses a compound collagen electuary with a high absorption and utilization rate and a preparation method thereof. The method comprises the following steps: selecting the collagen of cultivated animals or marine fishes; simultaneously adding the auxiliary components, such as trypsinase, astragalus extract, soyabean protein peptides and probiotic bacteria; fully utilizing the mutual effects of the substances to improve the utilization and absorption rate of the collagen; and decomposing macromolecular proteins which are not easy to absorb by human bodies in the collagen into micromolecular proteins which are easier to absorb by human bodies by adding the trypsase, wherein, the probiotic bacteria can improve the ecological balance of intestinal flora in human bodies; and bacillus subtilis and bacillus natto can change the intestinal flora ecology, and lead the functions of a digestive tract to be normalized. By utilizing the invention, the dosage of the collagen can be reduced by half to achieve beautification and healthcare effects, and meanwhile, the phenomena, such as constipation and the like caused by the fact that a large amount of collagen is taken by human bodies can be avoided.

The invention relates to a probiotic feed additive for Chinese softshell turtles and a preparation method of the probiotic feed additive. The probiotic feed additive is prepared from probiotics and carrier fillers, wherein the carrier fillers are sterilized wheat bran, and the probiotics include bacillus subtilis, bacillus natto, Lactobacillus casei and candida utilis with the ratio of (0.4-0.6):(0.4-0.6):(0.8-1.2):(0.8-1.2), and the probiotics are added in the aseptic carrier fillers to prepare probiotic powder. The probiotic feed additive can improve the growth performance of the Chinese softshell turtles, reduce the food coefficient, improve the immunological function and reduce the diseases, has the advantages of low cost, no pollution, no drug resistance, no residue and the like, and is environmentally friendly.

The invention aims to provide a method for preparing a compound bacterium agent for livestock breeding. The method comprises the following steps: fermenting bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus and clostridium butyricum, adding a carrier, performing plate-frame pressure filtration, adding a protective agent for emulsifying and spray drying, and finally, matching according to effective bacteria numbers of the five strains. The method is simple in process and easy to operate, the carrier and protective agent adopted in the preparation process are edible components, the safety of the bacterium agent can be guaranteed, and a reasonable proportion among the five strains can be guaranteed in the method, so that the strongest synergistic effect is achieved, and the method is suitable for large-scale popularization and application.

The invention discloses a preparation method for an active carbon deodorant and application of the active carbon deodorant in removal of fish meal processing odor. The preparation method comprises the following steps of: inoculating at least one of bacillus subtilis, yeast, bacillus natto and rhizopus into a mixed solution containing ferrous chloride, zinc sulfate and glucose, adding active carbon particles with the particle size of 10-30 meshes, soaking for 10 to 30 minutes, taking the active carbon particles out, and draining for 5 to 10 minutes to obtain the active carbon deodorant. The ferrous chloride, the zinc sulfate, the glucose and microorganisms are adsorbed in the active carbon deodorant, the ferrous chloride, the zinc sulfate and the glucose contribute to growth and reproduction of the microorganism and have synergism for deodorizing; the microorganisms can degrade odor adsorbed by the active carbon particles, so that the effect of adsorbing the odor is good, the adsorbed odor can be degraded, and a problem that the active carbon is required to be replaced or desorbed after adsorption saturation is solved; meanwhile, a problem of secondary pollution during desorption of the active carbon is solved, and the active carbon deodorant has the deodorizing rate of over 99 percent when applied to removing the fish meal processing odor.