Composite bacillus spp. microecological preparation and preparation method thereof

A technology of composite bacillus and microecological preparations, which is applied in the field of solid-state fermentation of composite bacillus to produce microecological preparations and its preparation, and can solve the problem of high production cost of liquid fermentation

Inactive Publication Date: 2012-01-04
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention overcomes the defect of relatively high production cost of liquid fermentation in the preparation of microbia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The preparation method of the composite bacillus probiotics proposed by the present invention comprises the following steps:

[0022] (1) Inoculate the slanted seeds of the composite Bacillus test tube into the liquid fermentation medium for fermentation;

[0023] Among them, the liquid fermentation medium is: 15-25g of glucose, 50-100g of bean cake powder, 5-10g of corn steep liquor, and make up to 1L with water. pH value 6.8-7.2, sterilized at 121°C for 15-25min. The fermentation temperature in the liquid fermentation is 30°C-37°C, the slant of the compound bacillus seed test tube is inoculated in the triangular flask containing the liquid medium, placed in a shaker, and the shaker speed is 150-250r / min. . The compound bacillus seed contains any two or more of Bacillus subtilis, Bacillus licheniformis, Bacillus natto, Bacillus coagulans, Bacillus lentus and Bacillus pumilus.

[0024] (2) Inoculate the compound Bacillus seeds after liquid fermentation in step (1) in...

Embodiment 1

[0028] Embodiment 1, the preparation of compound bacillus liquid seed

[0029] First prepare the seed medium of Bacillus, glucose 20g, bean cake powder 70g, corn steep liquor 7g, water 1L, pH value 7.0, 0.1 MPa, sterilize for 20min. The slant of the Bacillus subtilis test tube, the slant of the Bacillus licheniformis test tube and the slant of the Bacillus natto test tube were respectively inoculated into 20 250mL Erlenmeyer flasks equipped with the above seed medium, and each Erlenmeyer flask was inoculated with these three strains respectively. In a constant temperature shaker at a temperature of 37° C., set the rotation speed of the shaker at 220 r / min, and cultivate for 24 hours.

Embodiment 2

[0030] Embodiment 2, compound bacillus solid-state fermentation

[0031] The seeds prepared in Example 1 were used to inoculate into a solid-state fermentation workshop with a constant temperature device and ventilation device. The solid-state fermentation medium is 300g of sawdust, 2400g of bran, 300g of soybean meal powder, 30g of calcium carbonate, 1.5L of water, and pH7.0. The medium was sterilized at 121°C for 30min. The inoculum size is 5% (the ratio of the seed volume to the mass of the solid medium), the fermentation temperature is 37°C, the aeration rate is 0.5m2 / h at the beginning of fermentation, the aeration rate increases to 1m2 / h after 12 hours of fermentation, and the aeration rate increases after 24 hours of fermentation to 1.5m 2 / h, fermentation ended in 36 hours. Then the temperature rises to 40°C, and the ventilation rate is 2m2 / h. After 24 hours, the water content of the final product is less than 10%, and the number of viable bacillus bacteria reaches ...

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Abstract

The invention relates to microecological preparations, and specifically relates to a microecological preparation produced through composite bacillus spp. solid state fermentation, and a preparation method thereof. The composite bacillus spp. microecological preparation provided by the invention is prepared from any two or more than two of bacillus subtilis, blichenif irmis, B.natto, B.licoglans, B.lentus and B.pumilus. The materials are mixed, and are processed through solid state fermentation, such that a composite preparation is obtained. With the composite bacillus spp. microecological preparation provided by the invention, pollutants such as organics, nitrogen and phosphorus in a cultivation pond can be effectively decomposed, and a water body environment of intensive aquaculture can be repaired. According to the invention, the microecological preparation is produced from composite bacillus spp., which is prepared with cheap culture mediums and the solid state fermentation technology. Therefore, the preparation and the method provided by the invention assist in the popularization and application of microbial preparations.

Description

technical field [0001] The invention relates to a micro-ecological preparation, in particular to a micro-ecological preparation produced by solid-state fermentation of compound bacillus and a preparation method thereof. Background technique [0002] my country is a big producer and exporter of aquatic products. Due to the reduction of wild fishery resources, aquatic products mainly rely on the intensive aquaculture industry model. Intensive farming is a high-density farming system characterized by high input of artificial bait. Compared with the traditional farming model, intensive farming has the advantages of high output per unit of water body, short breeding cycle, and high labor efficiency. However, in this intensive and high-density culture, the amount of residual bait and excrement exceeds the natural decomposition ability of microorganisms in the culture pond, which often breaks the micro-ecological balance of the water body, resulting in the increase of organic mat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10C12R1/07
Inventor 常忠义高红亮王亭芳赵云龙
Owner EAST CHINA NORMAL UNIV
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