Fermented traditional Chinese medicine preparation capable of reducing diarrhea ratio of piglets
A traditional Chinese medicine preparation, piglet diarrhea technology, applied in animal feed, animal feed, application and other directions, can solve the problems of intestinal damage, gastrointestinal enzyme level and absorption capacity decline, etc., to achieve improved taste, improved drug efficacy, and easy for animals to take. Effect
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[0011] 1. Preparation of compound probiotic seed liquid:
[0012] 1) Activation of the stored slant strains, cultured in shake flasks respectively, Bacillus polymyxa was cultured in LB medium, pH 7.3, autoclaved at 120° C. for 30 minutes, and cultured at 37° C. for 15 hours. Lactobacillus inulin and heat-resistant Bacillus use PDA medium, pH 7.5, autoclaved at 120°C for 30 minutes, and cultivated at 30°C for 20 hours;
[0013] 2) Primary liquid expansion culture (50L tank) of Bacillus polymyxa, Lactobacillus inulin, and heat-resistant Bacillus; inoculum size 15%;
[0014] 3) Secondary liquid expansion culture (500L tank) of Bacillus polymyxa, Lactobacillus inulin, and heat-resistant Bacillus; inoculum size 15%;
[0015] 4) Mix the seed liquid of Bacillus polymyxa, Lactobacillus inulin, and heat-resistant Bacillus according to the following ratio: Bacillus polymyxa 10%-20%, Lactobacillus inulin 30%-60%, heat-resistant Bacillus Bacillus 30%-60%;
[0016] 2. Preparation method...
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