Rapid detection method for content of aflatoxin in brown rice based on FT-NIR technology

A technology of aflatoxin and detection method, which is applied in the field of grain quality detection, and can solve problems such as threatening human health and losing the protective effect of rice husk

Inactive Publication Date: 2015-12-16
NANJING UNIV OF FINANCE & ECONOMICS
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Problems solved by technology

However, due to the loss of the protective effect of rice husk, brown rice is more susceptible to fungal contamination during storage and transportation and produces aflatoxin, which seriously threatens human health.

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  • Rapid detection method for content of aflatoxin in brown rice based on FT-NIR technology
  • Rapid detection method for content of aflatoxin in brown rice based on FT-NIR technology
  • Rapid detection method for content of aflatoxin in brown rice based on FT-NIR technology

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Embodiment Construction

[0067] In order to better understand the technical content of the present invention, specific embodiments are given together with the attached drawings for description as follows.

[0068] Aspects of the invention are described in this disclosure with reference to the accompanying drawings, which show a number of illustrated embodiments. Embodiments of the present disclosure are not necessarily intended to include all aspects of the invention. It should be appreciated that the various concepts and embodiments described above, as well as those described in more detail below, can be implemented in any of numerous ways, since the concepts and embodiments disclosed herein are not limited to any implementation. In addition, some aspects of the present disclosure may be used alone or in any suitable combination with other aspects of the present disclosure.

[0069] combine figure 1 The realization process of the rapid detection method of aflatoxin in the brown rice of the present...

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Abstract

The invention provides a rapid detection method for the content of aflatoxin in brown rice based on FT-NIR technology. The method comprises the following steps: step 1, sample preparation: a step of collecting brown rice sample with different Aspergillus flavus invasion degrees, crushing the brown rice samples to obtain sample powders and refrigerating the sample powders for detection; step 2, spectral detection: a step of collecting diffuse reflection spectral information of the sample powders by using a Fourier transform near-infrared spectrometer and determining the contents of aflatoxin B1, B2, G1 and G2 and a total amount thereof in the sample powders by using a multifunctional column purification-high performance liquid chromatography-fluorescence method; step 3, spectral pretreatment: a step of pretreating original spectral information of the sample powders obtained in the previous steps so as to eliminate interference; step 4, quantitative forecasting and analysis: a step of analyzing the pretreated spectral information by using a stepwise multivariate linear regression method (SMLR) and establishing a stoichiometrical model of the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof in the sample powders and the spectral information of the samples; and step 5, rapid determination: a step of outputting the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof by using the established model on the basis of the spectral information of to-be-detected brown rice.

Description

technical field [0001] The invention relates to the technical field of grain quality detection, in particular to a method based on FT-NIR technology to realize simultaneous detection of aflatoxin B in brown rice 1 , B 2 , G 1 , G 2 and a rapid detection method for its total level. Background technique [0002] Grains are rich in carbohydrates, fats, proteins and inorganic salt nutrients, and are important strategic materials related to the national economy and people's livelihood. However, under suitable moisture and temperature conditions, grains are extremely prone to mold and deterioration. According to reports, the post-harvest grain loss caused by mildew in my country is as high as 21 million tons each year, accounting for 4.2% of the total grain output in the country, which is more than four times the annual increase in grain output. [0003] There are about 200 kinds of common molds in grains, and there are more than 300 kinds of toxins produced by mold metabolis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
Inventor 沈飞吴启芳
Owner NANJING UNIV OF FINANCE & ECONOMICS
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