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Method for separating luteolin 5‑o‑β‑glucoside from longan meat

A technology of luteolin and glucoside, which is applied in the field of separation of luteolin 5-O-β-glucoside, can solve the problems of not obtaining luteolin 5-O-β-glucoside and achieve rapid and efficient separation Effect

Active Publication Date: 2018-03-20
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, no one has isolated luteolin 5-O-β-glucoside from longan pulp

Method used

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  • Method for separating luteolin 5‑o‑β‑glucoside from longan meat
  • Method for separating luteolin 5‑o‑β‑glucoside from longan meat
  • Method for separating luteolin 5‑o‑β‑glucoside from longan meat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Take 500g of fresh longan pulp, add 80% acetone pre-cooled at 4°C according to the mass-volume ratio of 1:2, use a Philips mixer to break the tissue, and then use an XHF-D homogenizer to homogenize in an ice-water bath at a speed of 5000rpm. Homogenize for 5 minutes, centrifuge at 5000rpm for 5min after homogenization, and take the supernatant. The pomace was extracted once more. The centrifuged supernatants were combined twice, concentrated in vacuo to about 40 mL, and the concentrated longan pulp extract was refrigerated at -80°C for later use.

[0027] (2) The longan pulp extract concentrate is first adopted to fill the HPD-826 type macroporous resin with a diameter of 3.5cm and a long 40cm glass column to separate, wash with 2L ultrapure water and 2L 95% ethanol solution (v / v) respectively take off. The organic phase eluate was collected, and the organic phase eluate was concentrated in vacuo.

[0028] (3) The concentrated solution of the organic phase is fur...

Embodiment 2

[0038] (1) Take 500g of fresh longan pulp, add 80% acetone pre-cooled at 4°C according to the mass volume ratio of 1:2, use a Philips mixer to break the tissue, and then use an XHF-D homogenizer to homogenize in an ice-water bath at a speed of 5500rpm. Homogenize for 6 minutes, centrifuge at 4500rpm for 4min after homogenization, and take the supernatant. The pomace was extracted once more. The centrifuged supernatants were combined twice, concentrated in vacuo to about 40 mL, and the concentrated longan pulp extract was refrigerated at -80°C for later use.

[0039] (2) The longan pulp extract concentrate is first adopted to fill the HPD-826 type macroporous resin with a diameter of 3.5cm and a long 40cm glass column to separate, wash with 2L ultrapure water and 2L 95% ethanol solution (v / v) respectively take off. The organic phase eluate was collected, and the organic phase eluate was concentrated in vacuo.

[0040] (3) The concentrated organic phase is further purified wi...

Embodiment 3

[0043] (1) Take 500g of fresh longan pulp, add 80% acetone pre-cooled at 4°C according to the mass volume ratio of 1:2, use a Philips mixer to break the tissue, and then use an XHF-D homogenizer to homogenize in an ice-water bath at a speed of 4500rpm. Homogenize for 6 minutes, centrifuge at 4500rpm for 6min after homogenization, and take the supernatant. The pomace was extracted once more. The centrifuged supernatants were combined twice, concentrated in vacuo to about 40 mL, and the concentrated longan pulp extract was refrigerated at -80°C for later use.

[0044] (2) The longan pulp extract concentrate is first adopted to fill the HPD-826 type macroporous resin with a diameter of 3.5cm and a long 40cm glass column to separate, wash with 2L ultrapure water and 2L 95% ethanol solution (v / v) respectively take off. Collect the eluate from the organic phase, and concentrate the eluate from the organic phase in vacuo to obtain a concentrated solution.

[0045] (3) The concentr...

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Abstract

The invention discloses a method for separating luteolin 5-O-β-glucoside from longan flesh, comprising the following steps: (1) extracting longan flesh with acetone to obtain an acetone extract; The macroporous resin separation column is eluted successively with ultrapure water and 95% ethanol solution, and the ethanol solution is collected and concentrated until the ethanol is completely recovered; (3) the concentrated solution is put on a polyamide resin column, followed by water and a volume fraction of 10 %, 20% ethanol for gradient elution, and then use ethanol with a volume fraction of 25-35% for elution, and collect the eluent; (4) inject the eluent of 25-35% ethanol into the high-performance liquid chromatograph , eluted with methanol-water, collected fractions containing luteolin 5-O-β-glucoside, concentrated, and dried to obtain luteolin 5-O-β-glucoside. The method has the advantages of simple process, easy control of conditions, and easy industrialization, which provides convenience for its wide-ranging use.

Description

technical field [0001] The invention relates to a method for separating phenolic compounds from longan meat, in particular to a method for separating luteolin 5-O-β-glucoside from longan meat. Background technique [0002] Longan is known as the "sacred fruit among fruits" and has the reputation of "Southern Ginseng". It has a long history of cultivation in my country and is mainly distributed in Fujian, Guangxi, Guangdong, Sichuan and Taiwan Provinces. It is a characteristic bulk fruit in southern my country, of which Guangdong Province The longan planting area is 129,000 hectares, with an annual output of 554,800 tons, which plays an important role in local agricultural and economic development. Longan pulp is not only nutritious, but also has high medicinal efficacy. "Compendium of Materia Medica" records that "lychee is the most expensive food, and longan is the best resource", and it is believed that longan meat has the effect of "appetizing and invigorating the spleen,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 遆慧慧张名位魏振承张瑞芬张雁唐小俊邓媛元刘磊马永轩郭锦欣
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI