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Neovison vison klebsiella peneumoniae

A Klebsiella and mink technology, applied in the field of microorganisms, can solve problems such as increased drug resistance and difficult clinical treatment, and achieve the effect of strong toxicity

Inactive Publication Date: 2015-12-30
王贵升
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the abuse of antibiotics, the drug resistance of the bacteria has been continuously enhanced, and the emergence of multi-drug resistant strains has brought great difficulties to clinical treatment.

Method used

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  • Neovison vison klebsiella peneumoniae
  • Neovison vison klebsiella peneumoniae
  • Neovison vison klebsiella peneumoniae

Examples

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Embodiment 1

[0029] 1. Bacteria isolation and purification

[0030] Using a scalpel aseptic operation, open the chest and abdomen of the dead mink to expose the main organs. Use scissors to make a "well" shape to cut open the liver of a dead mink to fully expose its internal tissues; dip a sterile disposable cotton swab into the liver and then mark the urinary tract bacteria coloring medium with "Zig" Line, mark the petri dish with bacteria, put it into the biochemical incubator, and observe the growth of bacteria after 12 hours of constant temperature cultivation at 37°C. Pick a single blue-gray colony (Klebsiella pneumoniae grows as a blue-gray colony on the urinary tract bacteria chromogenic medium), and streak it on the tryptone soy agar medium to purify the colony. If the bacterial colony is still impure after a pure culture, continue to pick a single colony for bacterial colony purification until a pure colony grows.

[0031] 2. PCR identification

[0032] Primers: According to the spec...

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Abstract

The invention relates to a neovison vison klebsiella peneumoniae, and belongs to the technical field of microorganisms. The neovison vison klebsiella peneumoniae is named as a WMOK strain with the preservation number of CGMCC NO.11222, belongs to the K2 type, and is gram-negative (G-), facultative anaerobic, atrichous and asporous, has an obvious capsule, and besides, most strains have fimbriae and are rod-shaped with finely rounded ends; the WMOK strain has relatively high toxicity, has a half lethal dose (HLD) of 1.8 CFU to mice, and further has an HLD of 15 CFU to neovison vison. According to the neovison vison klebsiella peneumoniae, provided by the invention, separation and identification of the strain provide a certain foundation for further deep development of correlational studies on and control over neovison vison klebsiella peneumoniae.

Description

technical field [0001] The invention relates to a strain of mink Klebsiella pneumoniae, which belongs to the technical field of microorganisms. Background technique [0002] Klebsiella pneumoniae (Klebsiellapneumoniae) belongs to Enterobacteriaceae, Klebsiella genus, Gram-negative (G - ). Facultatively anaerobic, without flagella and spores, with obvious capsules, most strains have pili. Klebsiella pneumoniae has K (capsule) antigen and O (cell) antigen, the former is lipopolysaccharide, and the latter is polysaccharide. K antigen can be used to identify bacterial types. According to the typing of capsular polysaccharide (K antigen), Klebsiella pneumoniae can be divided into 82 K serotypes, among which K1, K2 and K5 serotypes are closely related to severe infectious diseases in humans and animals. The bacterium was first isolated from the lung tissue of a patient with lobar pneumonia by Friediander in 1883. This bacterium is a common pathogenic bacterium of hospital-acqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/22
CPCC12N1/20C12N1/205C12R2001/22
Inventor 王贵升王金宝田夫林单虎
Owner 王贵升
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