Method for inducing DC-CIK (dendritic cell-cytokine induced killer) cells by adopting cryopreserved cord blood cells
A technology of DC-CIK and blood cells, which is applied in the field of joint preparation of DC-CIK cells, can solve the problems of few hematopoietic stem cells and low induction efficiency, and achieve the effect of low cost, high induction efficiency and good repeatability
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[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
[0027] The method for inducing DC-CIK cells from frozen umbilical cord blood of the present invention is a method for co-cultivating DC-CIK cells, adopts multi-cell induction factors, and has a higher induction rate, in order to further clarify the biological characteristics of DC-CIK cells Provide a research platform.
[0028] The method for inducing DC-CIK cells from cryopreserved umbilical cord blood in this embodiment includes steps S101 to S106 for inducing DC cells.
[0029] S101. Preparation of human umbilical cord blood mononuclear cells: the umbilical cord blood is collected, and the umbilical cord blood mononuclear cells are prepared by hydroxyethyl starch precipitation.
[0030] S102. Cryopreservation and resuscitation of umbilical cord blood cells: the umbilical cord blood mononuclear cells prepared in step S101 were cool...
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