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Method for inducing DC-CIK (dendritic cell-cytokine induced killer) cells by adopting cryopreserved cord blood cells

A technology of DC-CIK and blood cells, which is applied in the field of joint preparation of DC-CIK cells, can solve the problems of few hematopoietic stem cells and low induction efficiency, and achieve the effect of low cost, high induction efficiency and good repeatability

Pending Publication Date: 2015-12-30
姚静
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the peripheral blood of healthy people is used to induce and culture DC-CIK. Since there are few hematopoietic stem cells in the peripheral blood, the induction efficiency is low.

Method used

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  • Method for inducing DC-CIK (dendritic cell-cytokine induced killer) cells by adopting cryopreserved cord blood cells
  • Method for inducing DC-CIK (dendritic cell-cytokine induced killer) cells by adopting cryopreserved cord blood cells
  • Method for inducing DC-CIK (dendritic cell-cytokine induced killer) cells by adopting cryopreserved cord blood cells

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0027] The method for inducing DC-CIK cells from frozen umbilical cord blood of the present invention is a method for co-cultivating DC-CIK cells, adopts multi-cell induction factors, and has a higher induction rate, in order to further clarify the biological characteristics of DC-CIK cells Provide a research platform.

[0028] The method for inducing DC-CIK cells from cryopreserved umbilical cord blood in this embodiment includes steps S101 to S106 for inducing DC cells.

[0029] S101. Preparation of human umbilical cord blood mononuclear cells: the umbilical cord blood is collected, and the umbilical cord blood mononuclear cells are prepared by hydroxyethyl starch precipitation.

[0030] S102. Cryopreservation and resuscitation of umbilical cord blood cells: the umbilical cord blood mononuclear cells prepared in step S101 were cool...

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Abstract

The invention discloses a method for inducing DC-CIK (dendritic cell-cytokine induced killer) cells by adopting cryopreserved cord blood cells. The method comprises following steps: (1), resuscitating the cryopreserved cord blood cells and performing adherent culture on the cryopreserved cord blood cells in a culture flask; (2), preparing DCs; (3), preparing CIK cells. The method for inducing the DC-CIK cells by adopting the cryopreserved cord blood cells has the advantages that inducing and enriching processes are simple and easy, the period is short, the cost is low, the induction efficiency is high, and activity of enriched cells is high.

Description

technical field [0001] The invention relates to a joint preparation method of DC-CIK cells, in particular to a method for inducing DC-CIK cells by frozen umbilical cord blood cells. Background technique [0002] In 1973, Steiman and Cohn first isolated a type of white blood cell from the spleen that was different in shape and function from granulocytes, macrophages, and lymphocytes, because its cell membrane protruded outwards, forming a membranous tree similar to the axons of nerve cells dendritic cells (DCs). DC cells are known to have the strongest function in vivo and the only professional antigen-presenting cells that can activate resting T cells. They are the central link in initiating, regulating and maintaining immune responses. By activating and culturing a large number of DC cells loaded with tumor antigens in vitro, when the number of cells reaches a certain number, they will be reinfused back to the patient, which can induce a strong anti-tumor immune response i...

Claims

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Application Information

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IPC IPC(8): C12N5/0784C12N5/0783
CPCC12N5/0639C12N5/0646C12N2501/22C12N2501/2301C12N2501/2302C12N2501/24C12N2501/25C12N2501/998C12N2502/1121C12N2502/1164
Inventor 姚静
Owner 姚静
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