Preparation method of antioxidant peptide chain from crab shell leftovers
A technology of antioxidant peptides and scraps, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., and can solve the problems of unreported high-activity antioxidant peptides.
Active Publication Date: 2020-10-20
ZHEJIANG OCEAN UNIV
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[0004] The applicant found that using crab shell leftovers as raw materials, the process research on the preparation of antioxidant peptides from crab shell leftovers by enzymatic hydrolysis technology is in a blank stage, and the preparation of highly active antioxidant peptides and their applications using crab shell leftovers as materials It has not been reported
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[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0022] A method for preparing antioxidant peptide chains from crab shell leftovers, the preparation process is as follows: crab shell leftovers-defatting-enzymolysis-enzymolyzate-ultrafiltration-macroporous resin purification-anion exchange chromatography-gel filtration layer Analysis - Reverse Phase High Performance Liquid Chromatography - Antioxidant Peptides.
[0023] The specific steps are:
[0024] 1) Pretreatment of crab shell scraps: Portunus trituberculatus ( Portunus trituberculatus ) crab shell scraps, add isopropanol according to the solid-to-liquid ratio of 1g: 8mL, degrease at 45°C for 8 h, then centrifuge at 9000 rpm for 10 min to remove isopropanol, and collect the solid matter of the defatted crab shell scraps.
[0025] 2) Enzymatic hydrolysis of crab shell leftovers: Add the above-mentioned defatted crab shell leftover soli...
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Abstract
The invention discloses a method for preparing an antioxidative peptide chain by utilizing crab shell offal. The method comprises the following steps: carrying out degreasing as well as enzymolysis by utilizing neutral protease and papayotin to prepare an enzymolysis solution; conducting ultrafiltration, macroporous resin purification, anion exchange resin chromatography, gel column chromatography, and reversed-phase high performance liquid chromatography separation and purification on the enzymolysis solution to obtain the antioxidative peptide chain Ile-Trp-Met-Glu-Cys-Asn-Trp of which the molecular mass measured by ESI-MS is 979.15 Da. The prepared peptide chain has not only a good function of scavenging DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals, but also a good function of inhibiting lipid peroxidation.
Description
technical field [0001] The invention relates to a method for preparing protein antioxidant peptides, in particular to a method for preparing antioxidant peptide chains from crab shell leftovers. Background technique [0002] The output of aquatic products in China accounts for about one-third of the world's total output, but the processing rate is about 30%, which is far behind the 80% in the United States and other countries. The processing and comprehensive utilization technologies of aquatic products need to be further improved. At present, the utilization rate of by-products of aquatic product processing in my country is relatively low, while foreign countries have achieved effective classification and utilization according to the chemical composition and biochemical characteristics of by-products, and the processing output value is even several to dozens of times higher than that of fish. During the processing of shrimp and crab aquatic products, a large amount of lefto...
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IPC IPC(8): C12P21/06C07K1/34C07K1/20C07K1/18
Inventor 曲有乐杨立业徐银峰王斌陈荫孙坤来
Owner ZHEJIANG OCEAN UNIV