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Method for extracting total saponins from radix platycodonis and available for industrial production

A technology of total saponins and platycodon, which is applied in the field of preparation of total saponins extracted from platycodon, can solve the problems of unfavorable industrial production, complicated operation, large consumption, etc., and achieve the effect of easy industrial large-scale production, high separation efficiency and high yield

Inactive Publication Date: 2016-01-06
CHENGDU AIBIKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main active ingredient of platycodon is platycodon saponin. The traditional preparation method of platycodon platycodon is high in cost, consumes a large amount of reagents such as acetone and ether, and is complicated to operate, which is not conducive to industrial production. Therefore, it is necessary to find a new extraction and separation method to make it suitable. in industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method for extracting total saponins from Platycodon grandiflorum which can be industrially produced, comprising the following specific process steps:

[0021] 1) Take the bellflower medicinal material, and grind the bellflower medicinal material into a powder of 10-100 mesh;

[0022] 2) adding water or 50-85% ethanol to the bellflower powder obtained in step 1), adjusting the solution to a pH value of 10-12 and then extracting to obtain an extract;

[0023] 3) Put the extract on a macroporous basic anion exchange column, wash it with alkaline water and pure water first, discard the washing solution, then elute with 50-85% ethanol, and concentrate the eluent to obtain the total saponins of Platycodon grandiflorum;

[0024] Wherein, the macroporous basic anion exchange column in step 2) is selected from macroporous weakly basic anion D392 exchange column, macroporous weakly basic anion D380 exchange column, macroporous weakly basic anion D382 exchange colum...

Embodiment 2

[0026] This embodiment is further optimized on the basis of the above embodiments. In order to further better realize the present invention, in the step 1), NaOH, KOH, Na 2 CO 3 or NaHCO 3 Adjust the pH of the solution.

Embodiment 3

[0028] This embodiment is further optimized on the basis of any of the above embodiments. In order to further better realize the present invention, the preparation process of the extract described in step 1) is as follows:

[0029] 1-1) adding bellflower powder to water or 50-85% ethanol to obtain a solution, the ratio of the powder to water or 50-85% ethanol is 1:3-5 by weight;

[0030] 1-2) Adjust the solution to an alkaline solution with a pH value of 10-12, heat or ultrasonically extract the alkaline solution for 2-4 times, combine the extracts, and filter; further remove impurities from the filtrate, and use alcohol precipitation for the water extract Impurities are removed, and the alcohol extract is removed by water precipitation to obtain a clear liquid after removal of impurities.

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PUM

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Abstract

The invention discloses a method for extracting total saponins from radix platycodonis and available for industrial production. The method specifically includes the technical steps: 1) grinding radix platycodonis into 10-100-mesh powder; 2) adding radix platycodonis powder obtained at the step 1) into water or 50-85% ethyl alcohol, adjusting pH value of solution to 10-12, and extracting to obtain extract liquid; 3) feeding the extract liquid to a macroporous alkaline anion exchange column, flushing with alkaline water and pure water, abandoning flush fluid, eluting with 50-85% ethyl alcohol, and concentrating elution liquid to obtain total saponins. Since a preparation mode available for industrial production is adopted for extraction of the total saponins, extraction efficiency can be effectively improved while yield of the total saponins is increased.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a preparation method for extracting total saponins from Platycodon grandiflorum which can be industrialized. Background technique [0002] Bellflower (BalloonFlower), also known as burden flower, bell flower, mitral flower, is a perennial herbaceous plant with a stem height of 20-120 cm, usually glabrous, occasionally densely covered with short hairs, unbranched, with very few upper branches. The leaves are all in whorls, some are in whorls to all are alternate, sessile or have a very short stalk, the leaves are ovate, ovate-elliptic to lanceolate, the leaves are ovate or ovate-lanceolate, the flowers are dark blue or dark blue Purple and white, it can be used as an ornamental flower; its root can be used as medicine, which has the functions of relieving cough and expectorant, clearing the lung, and draining pus. It is commonly used in traditional Chinese medicine. It...

Claims

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Application Information

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IPC IPC(8): A61K36/346
Inventor 何金蓉
Owner CHENGDU AIBIKE BIOTECH