Composite enzyme for pulp making and its preparation method and use method

A composite enzyme and pulp technology, applied in textile and papermaking, fiber raw material processing and other directions, can solve the problems of difficult preservation of biological enzymes, unfavorable slurry purification, difficulty in effect control, etc., to improve the pretreatment effect, prolong the storage time, and produce low cost effect

Active Publication Date: 2021-06-25
秾源科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological enzymes used in the pretreatment step of biopulping are cellulase, hemicellulase, lignin degrading enzyme and pectinase. Will choose to directly use industrially produced biological enzymes for production, but the direct use of industrially produced biological enzymes has difficulties in recovering the enzymes used, huge usage, increased production waste, and is not conducive to further purification of the slurry. At the same time, there are enzymes in the biological enzyme treatment. Low efficiency, difficult effect control, difficult storage of biological enzymes, and high requirements for use

Method used

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  • Composite enzyme for pulp making and its preparation method and use method
  • Composite enzyme for pulp making and its preparation method and use method
  • Composite enzyme for pulp making and its preparation method and use method

Examples

Experimental program
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Effect test

experiment example 1

[0053] This experiment example is in the present invention, the determination experiment of the enzymatic activity of the enzymatic activity of the obtained compound solution to the immobilization of cellulase, hemicellulase, lignin degrading enzyme and pectinase by the enzyme carrier prepared in the invention by the applicant .

[0054] Experimental materials: 10mg / mL enzyme carrier solution, 10mg / mL cellulase solution, 10mg / mL hemicellulase solution, 10mg / mL lignin degrading enzyme solution, 10mg / mL pectinase solution, Ionized water, pH=6 phosphate buffer.

[0055] making process:

[0056] 1. Take 10g of enzyme carrier solution, put it into the reaction kettle, add 30g of pH=6 phosphate buffer solution, 100g of raw material enzyme solution, place at 5°C for 24h, take it out and wash it with phosphate buffer solution to obtain a composite solution;

[0057] 2. Shake the compound enzyme solution obtained in step 1 at room temperature for 20 minutes, centrifuge for 5 minutes,...

experiment example 2

[0070] This experimental example is an experiment in which the applicant compares the effect of pulp pretreatment using pulping compound enzymes and other prior art pulp pretreatment effects in the present invention.

[0071] The pulp raw material used in this practical example is cotton stalk.

[0072] Preparation process a:

[0073] A kind of pulp is prepared by adopting the pretreatment process of the Chinese patent application (CN201710358393.7) in the prior art.

[0074] S1: Cut the cotton stalks into cotton stalk pieces, and transport them to the primary pretreatment immersion solution A. The primary pretreatment immersion solution A, in parts by weight, includes the following raw materials: 6 parts of alkylphenol polyoxyethylene ether, 0.5 parts of triethanolamine, 0.7 parts of stearic acid, 1 part of oleic acid, 1.2 parts of lauric acid, and 60 parts of water were soaked for 45 minutes under the action of ultrasonic waves, and then filtered to obtain the steeping mate...

experiment example 3

[0097] This experimental example is an experiment conducted by the applicant on the immobilization effect of hemicellulase and pectinase by using graphene oxide as a carrier in the present invention.

[0098] Experimental process A:

[0099] S1: Take 20kg of wood chips and place them in a sealed device, add a compound enzyme solution, the ratio of hemicellulase to pectinase in the graphene oxide compound enzyme solution is 5:1, and add deionized water to combine the enzyme solution with The slurry is mixed evenly, the dosage of hemicellulase is 5U / g absolute dry wood chips, and the consumption of pectinase is 1U / g absolute dry wood chips.

[0100] S2: react the solution obtained in step S1 at 45° C. for 1 hour, and slowly stir the material so that the material and the enzyme solution are fully mixed.

[0101] S3: heat the solution obtained in step S2 for 15 hours, and extract the prepared slurry.

[0102] S4: measuring the enzyme activity of the remaining reaction solution. ...

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Abstract

The invention provides a composite enzyme for pulp making, the composite enzyme includes an enzyme carrier and a biological enzyme, the enzyme carrier is graphene oxide, and the biological enzyme is cellulase, hemicellulase, and lignin degrading enzyme and one or more of the pectinases, the biological enzymes are attached and fixed on the enzyme carrier. The present invention adopts the above method, which can effectively immobilize biological enzymes on the graphene oxide carrier, fully utilizes the high proportion surface area and good biocompatibility of graphene oxide, and secondly, the functional groups rich in its surface are also acidic. The chemical properties of the graphene oxide can neutralize the alkaline ions in the solution, reduce the local pH value around the graphene oxide, and facilitate the activity of the biological enzymes attached to its surface. The use of graphene oxide to immobilize the biological enzymes makes the biological Most of the enzymes are kept in the liquid environment for secondary use, which improves the utilization efficiency of biological enzymes and reduces production waste.

Description

Technical field: [0001] The invention relates to the fields of pulping and papermaking and utilization of biomass resources, in particular to a composite enzyme for pulping and its preparation method and use method. Background technique: [0002] Lignocellulosic raw material, as an important and abundant biomass resource in nature, is an important production raw material in the current paper industry. In the prior art, the process of using microorganisms themselves or enzymes produced by microorganisms to pretreat fiber raw materials and then combine with corresponding pulping processes to produce pulp is called biopulping. For biopulping, its pretreatment step is the most important, because the use of bioenzyme pretreatment has little damage to the fibers in lignocellulosic raw materials, high fiber yield, energy saving, low production cost, and waste The discharge of liquid is small and the pollution load is low. The biological enzymes used in the pretreatment step of bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D21C5/00
Inventor 徐明邢立艳
Owner 秾源科技(北京)有限公司
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