A kind of bacillus licheniformis that replaces antibiotic feed additive and its application

A technology of Bacillus licheniformis and feed additives, which is applied in the field of agricultural biology, can solve the problems of no clear alternative antibiotic types and mechanism of action, low fermentation efficiency, and no specific types highlighted, so as to improve feed conversion rate, fermentation efficiency, Growth-promoting effect

Active Publication Date: 2018-07-10
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The above patent documents do not explicitly use Bacillus licheniformis, use Saccharomyces cerevisiae, Aspergillus niger, Aspergillus oryzae and other strains, adopt solid fermentation technology with low fermentation efficiency, and enhance the body's resistance by improving the non-specific immunity of animals To stimulate the ability of animals to improve animal production performance, there is no clear type and mechanism of action to replace antibiotics; some are combined with traditional Chinese medicines, organic acids, etc., without highlighting the specific types of microecological agents in patented products and the role they play in the product

Method used

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  • A kind of bacillus licheniformis that replaces antibiotic feed additive and its application

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Experimental program
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Embodiment 1

[0039] The above-mentioned Bacillus licheniformis (Bacillus licheniformis) SDZD02 was isolated, screened and identified from soil by the inventor. The bacterium was preserved in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms on April 2, 2015, and the preservation number is CGMCC NO.10682.

[0040] The bacterium is Gram-positive, straight rod-shaped, and forms a large number of spores after 48 hours of culture. The sporangia is columnar, subterminal, thin-walled, and the sporangia do not expand. ~0.8 μm×1.0~1.5 μm. It can grow at a temperature of 15°C to 55°C, and the optimum growth temperature range is 30°C to 40°C; the bacteria can grow well in the range of NaCl concentration of 0.1% to 8%, and when the NaCl concentration is 12%, the growth is limited Inhibition; the optimum growth pH range is 7.0-8.0; the physiological and biochemical characteristics of the bacteria are completely consistent with the typical characterist...

Embodiment 2

[0043] The application of Bacillus licheniformis SDZD02 described in Example 1 in the preparation of alternative livestock feed antibiotic additives.

[0044] The preparation method of the above-mentioned alternative animal husbandry feed antibiotic additive, the steps are as follows:

[0045] (1) Inoculate Bacillus licheniformis SDZD02 in the activation medium, activate and culture at 32°C for 34 hours, and obtain activated Bacillus licheniformis SDZD02 slant seeds;

[0046] Described activated solid culture medium, every liter of components is as follows:

[0047] 10 grams of peptone, 5 grams of yeast extract, 10 grams of sodium chloride, 15 grams of agar powder, distilled water to 1L.

[0048] The activated Bacillus licheniformis (Bacillus licheniformis) SDZD02, under aseptic conditions, was inoculated in a sterilized shake flask culture medium at 121°C. The rotation speed is 200rpm, and the incubation time is 16h to obtain the shake flask seed solution;

[0049] Shake f...

Embodiment 3

[0059] The preparation method of bacillus licheniformis as described in embodiment 2, the difference is that,

[0060] In described step (3), fermentation culture step is as follows:

[0061] The fermentor seed liquid that step (2) makes is inoculated on fermentation culture medium according to the inoculum amount of 10% by volume percentage to carry out fermentation culture, and at temperature 35 ℃, ventilation rate is 1: 1.1, tank pressure is 0.02MPa, stirring speed is Under the condition of 230rpm, ferment and cultivate for 16 hours, the spore rate is higher than 90% after testing; then the fermentation broth is treated at 80°C for 30 minutes, and the spore content is 2.5×10 10 FU / mL of fermentation broth

[0062] The components of the fermentation medium are as follows, all in parts by weight:

[0063] 4 parts of cornstarch, 3 parts of bean cake powder, 1 part of molasses, 0.1 part of sodium chloride, 0.1 part of ammonium sulfate, 0.3 part of manganese sulfate, 91.5 part...

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Abstract

The invention relates to a bacillus licheniformis which replaces the antibiotic feed additive and its application. A strain of Bacillus licheniformis (Bacillus licheniformis) SDZD02 was preserved on April 2, 2015 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing , the deposit number is CGMCC NO.10682. The present invention substitutes animal husbandry antibiotic feed additives through liquid submerged fermentation, plate-and-frame filter press, and boiling drying technology, and the number of effective viable bacteria is not less than 2.0×1010 CFU / g. It is used in modern animal husbandry to replace antibiotic feed additives, It has the characteristics of promoting growth, preventing and resisting diseases, improving feed conversion rate, no residue and no pollution.

Description

technical field [0001] The invention relates to a bacillus licheniformis and an application thereof, in particular to a bacillus licheniformis which can replace an antibiotic feed additive and an application thereof, and belongs to the field of agricultural biotechnology. Background technique [0002] Antibiotics have been used as antimicrobial growth promoters in animal husbandry for more than 60 years. As a growth promoter, antibiotics greatly reduce feeding costs, morbidity and mortality, and improve animal production performance, thereby significantly improving labor productivity and economic benefits of animal husbandry. However, the long-term use of antibiotics directly endangers human health and public health. Long-term low-dose feed antibiotics increase bacterial resistance and accelerate the production of "super bacteria"; feed drugs are discharged into the environment in the form of prototypes or metabolites along with animal excrement In addition, antibiotics dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10
Inventor 岳寿松王翠萍边斐张燕陈高葛海涛马德源刘国霞
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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