Method for extracting plant leaf anthocyanin
A plant leaf and extraction method technology, which is applied in the field of plant leaf anthocyanin extraction, can solve the problems of high extraction cost and long processing time, and achieve the effects of short processing time, reduced extraction cost, and reduced cost
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Embodiment 1
[0031] The extraction method of the plant leaf anthocyanin of the present embodiment, comprises the following steps:
[0032] 1) Collection and processing of plant leaf samples: In May 2015, in the campus of Northwest A&F University, the leaves of different ages of Prunus chinensis were collected. The leaves were randomly selected based on the visual characteristics, and the color of the leaves ranged from green, reddish to completely red , and healthy, without damage, collected 342 leaves of Ziye Plum, 1 sample for every 3 leaves, a total of 114 samples; the leaves of Ziye Plum were washed with water to remove impurities, blotted dry, and cut into 2 × 5mm long strip fragments;
[0033] 2) Take 10 g of the strip-shaped fragments described in step 1), add to 1L of acidified methanol (the volume ratio of hydrochloric acid and methanol solution is 0.837:100, the mass concentration of hydrochloric acid is 36%, and the volume content of methanol in methanol solution is 95% ) for 2...
Embodiment 2
[0048] The extraction method of the plant leaf anthocyanin of the present embodiment, comprises the following steps:
[0049] 1) At the beginning of April 2015, in the Peony Garden of Northwest Agriculture and Forestry University, 14 leaves of different varieties of peonies were collected in the early stage of flowering. The leaves were randomly selected based on visual characteristics. The color of the leaves ranged from green, reddish to completely red, and they were healthy. , No damage, 396 peony leaves were collected, 1 sample per 3 leaves, a total of 132 samples. Wash peony leaves with water to remove impurities, blot dry, and cut into 2×5mm strips;
[0050] 2) Take 20g of the strip-shaped fragments described in step 1), add to 1L of acidified methanol (the volume ratio of hydrochloric acid to methanol solution is 0.837:100, the mass concentration of hydrochloric acid is 36%, and the volume content of methanol in methanol solution is 95% ) for 2 extractions, each extrac...
Embodiment 3
[0053] The extraction method of the plant leaf anthocyanin of the present embodiment, comprises the following steps:
[0054] 1) Treatment of plant leaves: take the leaves of Ziye Prunus, wash them with water, remove impurities, blot them dry, and cut them into 2×5mm strip-shaped pieces;
[0055] 2) Get 15g of strip-shaped fragments described in step 1), add to 1L of acidified methanol (the volume ratio of hydrochloric acid and methanol solution is 0.837:100, the mass concentration of hydrochloric acid is 38%, and the volume content of methanol in methanol solution is 98% ) for 3 extractions, each extraction temperature is 30°C, and the time is 2.5h. After each extraction, it is centrifuged at 3000r / min, and the centrifuged supernatants are combined for 3 times and filtered to obtain anthocyanins. extract.
[0056] The extraction amount of anthocyanins in the extracted plant leaves was measured by a spectrophotometer, the specific operation method was the same as that in Exampl...
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