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Micropipe for blood grouping, one-piece ABO/RhD blood typing test card for blood grouping and preparation thereof

A detection card, an integrated technology, applied in the field of blood type detection, can solve the problems of cumbersome operation steps and low efficiency, and achieve the effect of reducing operation steps and improving efficiency

Active Publication Date: 2016-01-06
SHENZHEN DAKEWE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current ABO / RhD blood typing test card needs to add diluted red blood cells to perform the test, and the operation steps are cumbersome and inefficient

Method used

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  • Micropipe for blood grouping, one-piece ABO/RhD blood typing test card for blood grouping and preparation thereof
  • Micropipe for blood grouping, one-piece ABO/RhD blood typing test card for blood grouping and preparation thereof
  • Micropipe for blood grouping, one-piece ABO/RhD blood typing test card for blood grouping and preparation thereof

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preparation example Construction

[0040] The present invention also provides a method for preparing an integrated ABO / RhD blood typing test card, in a specific embodiment, comprising the following steps:

[0041] (1) prepare antibody diluent, the specific gravity of described antibody diluent is greater than serum separation gel and less than erythrocyte;

[0042] (2) Gel swelling: use physiological saline or the antibody diluent to swell the gel;

[0043] (3) Preparation of antibody working solution: anti-A monoclonal antibody, anti-B monoclonal antibody and anti-D monoclonal antibody were prepared by adding 7 volume parts of the antibody diluent for every 1 volume part of the antibody to prepare the antibody working fluid;

[0044](4) Preparation of specific gels: According to the ratio of adding 1.2-1.7ml of the antibody working solution per 1g of the swollen gel, they were respectively prepared into anti-monoclonal anti-A gels with IgM properties and anti-monoclonal anti-A gels with IgM properties. Anti-...

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Abstract

The invention discloses a micropipe for blood grouping, a one-piece ABO / RhD blood typing test card for blood grouping and a preparation thereof. The micropipe for blood grouping includes a gel, an antibody working solution and a serum separation gel from bottom to top in order. The antibody working solution comprises a blood group antibody and an antibody diluent which can make the blood group antibody maintain activity, and the serum separation gel having a specific gravity of 1.075-1.077 can perform thixotropy to turn the solid into the fluid in the presence of centrifugal force of 150 G to 300 G. The specific gravity of the antibody diluents is more than that of the serum separation gel and less than that of the erythrocyte. The one-piece ABO / RhD blood typing test card prepared by the micropipe can add peripheral blood directly to test, and has no need to test the blood sample after diluting the peripheral blood removed the erythrocyte by centrifuging. So that the sensitivity and specificity of the blood type test card are guaranteed, the operation steps of the traditional ABO / RhD blood typing test card are reduced, and the efficiency is improved.

Description

technical field [0001] The invention relates to blood type detection, in particular to a microtube for blood type detection, an integrated ABO / RhD blood type detection card and a preparation method. Background technique [0002] Since the discovery of ABO blood type, ABO blood type has been widely used in clinical practice. Accurate typing of ABO blood type is very important and is the basis and guarantee for safe blood transfusion. The Rh blood group system is an important blood group system second only to the ABO blood group system, and it is also the most complex system in the red blood cell blood group system. The antigens in the Rh blood group system include D, C, E, etc., and the D antigen has the strongest antigenicity. If the red blood cell surface contains D antigen, it is RhD positive, if it does not contain D antigen, it is RhD negative. After RhD negative patients receive RhD positive blood, at least 20% of patients will develop an immune response to D antigen, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 钟志荣周翔吴庆军
Owner SHENZHEN DAKEWE BIOENG
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