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Ported parallel plate flow chamber and methods for use thereof

A flow chamber and port technology, applied in stress-stimulated microbial growth methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of large-scale cultivation without providing multiple flow channel conditions or readout methods Activities and other issues

Active Publication Date: 2016-01-06
思安初有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these systems are currently commercially available for large-scale culture activities
There are a few companies on the market (e.g., Ibidi, Fluxion, Cellix, Cellasics, Integrated Biodiagnostics, and Glycotech) that sell some flow chambers based on parallel plate designs; however, most of them are based on small microfluidic channels and do not offer a variety of flow channels condition or readout method

Method used

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  • Ported parallel plate flow chamber and methods for use thereof
  • Ported parallel plate flow chamber and methods for use thereof
  • Ported parallel plate flow chamber and methods for use thereof

Examples

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example

[0086] The following examples provide illustrative embodiments. Those skilled in the art, in light of this disclosure and one's ordinary level of skill in the art, will appreciate that the following examples are intended to be illustrative only and that many variations, modifications and changes may be employed without departing from the scope of the disclosed subject matter.

example 1

[0088] Biomarker Analysis of Fluid Flow Regulated Cells

[0089] Differences in gene expression under two different shear stress conditions were tested in human aortic endothelial cells using a flow cell setup.

[0090] Table 1 shows the number of genes changed between human aortic endothelial cells exposed to a wall shear stress of 1.0 Pa for 20 hours compared to cells not exposed to flow. Cells were grown on collagen I-coated growth surfaces under static conditions (no flow) until confluence was reached. Cells were then exposed to fluid flow in the flow chamber at 0.2 or 1.0 Pa for 20 hours, or maintained in static culture for the same amount of time. After 20 hours, use AmbionMIRVANA TM The RNA Isolation Kit (Life Technologies, Foster City, CA, USA) isolated RNA from the cells and performed the analysis on Affymetrix PRIVIEW TM Array (Affymetrix Corporation, Santa Clara, California, USA) for analysis. Three experiments were performed for each condition, repeating the ...

example 2

[0096] Drug treatment of fluid flow preconditioned and statically cultured cells

[0097] PI103(3-[4-(4-morpholino)pyrido[3',2',4,5]furo[3,2-d] Pyrimidin-2-yl]phenol; CAS No. 371935-74-9), a flow cell setup was also used to test for gene expression differences in human aortic endothelial cells under different shear stress conditions.

[0098] Cells were grown on collagen I-coated growth surfaces and treated with 100 nM PI-103 under flow. For the 4 hour time point, cells were exposed to fluid flow for 16 hours and treated with PI-103 for the final 4 hours with flow. Cells were also treated with PI-103 throughout the duration of flow (20 hours). The data were generated using the extracted RNA as described in Example 1 and presented in Affymetrix PRIVIEW TM Array (Affymetrix) was used for analysis. Three experiments were performed for each condition, repeating the microarray analysis.

[0099] Table 2 shows the number of genes that were significantly different between pair...

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Abstract

Flow chambers are provided. In some embodiments, the flow chambers include an inner panel having at least one flow channel having an inlet / outlet opening on each end thereof formed therein, wherein the inlet / outlet openings are adapted to releasably receive a septum; one or more ports adapted to releasably receive a plug and for at least liquid communication with the at least one flow channel, and an outer frame that defines an outer portion of the at least one flow channel and that defines a perimeter of the flow chamber. In some embodiments, the flow chamber has overall dimensions of a standard multiwell plate and the at least one flow channel is located in a position that corresponds to a column location of the standard multiwell plate. Also provided are methods for producing the presently disclosed flow chambers and employing the same to assay biological features of cultured cells and / or tissues.

Description

[0001] Cross-references to related applications [0002] The subject matter of the present disclosure claims priority from US Provisional Patent Application Serial No. 61 / 791,770, filed March 15, 2013, the entire contents of which are hereby incorporated by reference. technical field [0003] The subject matter of the present disclosure relates generally to parallel plate flow cells and methods of using the same to examine the effects of different fluid flows on cells and their biological activity. In particular, the disclosed subject matter relates to devices on which cells and / or tissues can be cultured and examined in response to different fluid flow environments. Background technique [0004] In vitro cell culture is often performed as part of a variety of biological research and R&D projects. In its most common form, cell-based experiments are performed in petri dishes or flasks under static conditions (ie, those conditions in which no external force is applied to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/483G01N33/487C12M1/00
CPCB01L3/5025B01L2200/027B01L2200/0689B01L2300/044B01L2300/0822B01L2300/0829B01L2300/0877B01L2400/0487B01L2400/086G01N33/5023G01N33/5064C12M41/46G01N33/487B01L3/502B01L2200/025B01L2200/026B01L2300/0609B01L2300/0627C12M35/04C12Q1/6876C12Q2600/158
Inventor 克里斯蒂娜·D·林克罗伯托·D·谢菲尔德
Owner 思安初有限公司