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Application of total paeony glycosides to preparation of medicine for preventing or treating microwave radiation induced nerve cell injury, pharmaceutical composition and food

A technology of nerve cell damage and total glucosides of paeony, which is applied in the field of medicine, can solve the problems that research and development still need to be strengthened, and achieve the effect of reducing nerve cell damage, reducing content, and repairing nerve cells

Active Publication Date: 2016-01-13
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the research and development of drugs that can be used to prevent and treat nerve cell damage caused by microwave radiation still needs to be strengthened.

Method used

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  • Application of total paeony glycosides to preparation of medicine for preventing or treating microwave radiation induced nerve cell injury, pharmaceutical composition and food
  • Application of total paeony glycosides to preparation of medicine for preventing or treating microwave radiation induced nerve cell injury, pharmaceutical composition and food
  • Application of total paeony glycosides to preparation of medicine for preventing or treating microwave radiation induced nerve cell injury, pharmaceutical composition and food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Effect of total glucosides of paeony on changes in ROS content in H9c2 nerve cells induced by microwave radiation

[0063] 1. Culture induced PC12 cells

[0064] Take out the PC12 cell line with the characteristics of neurons and neurosecretory cells from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of 10% fetal bovine serum, 5% horse serum, and 100 units of penicillin / DMEM culture medium (dubecco'sminimallessentialmedium, DMEM) of 100 micrograms / ml of streptomycin, centrifuged at 1000 rpm for 10 minutes, discarded the supernatant, added 4 ml of the above-mentioned medium, pipet evenly, and sucked it into the culture bottle. Change medium every other day, 37°C, 5% CO 2 Cultivation and passage under conditions; after the above-mentioned cultured cell line was passed for 3 generations, it was induced to differentiate into neuron-like cells by using DMEM medium with 5 ng / ml NGF and 1...

Embodiment 2

[0084] Example 2 Effect of total glucosides of paeony on changes in ROS content in PC12 cells induced by microwave radiation

[0085] 1. Culture and induction of PC12 cells

[0086] Take out the PC12 cell line with the characteristics of neurons and neurosecretory cells from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of 10% fetal bovine serum, 5% horse serum, and 100 units of penicillin / DMEM culture medium (dubecco'sminimallessentialmedium, DMEM) of 100 micrograms / ml of streptomycin, centrifuged at 1000 rpm for 10 minutes, discarded the supernatant, added 4 ml of the above-mentioned medium, pipet evenly, and sucked it into the culture bottle. Change medium every other day, 37°C, 5% CO 2 Cultivation and passage under conditions; after the above-mentioned cultured cell line was passed for 3 generations, it was induced to differentiate into neuron-like cells by using DMEM medium with 5 ng / ml NGF an...

Embodiment 3

[0098] Example 3 Effect of total glucosides of paeony on changes in MDA content in PC12 cells induced by microwave radiation

[0099] 1. Culture and induction of PC12 cells

[0100] Take out the PC12 cell line with the characteristics of neurons and neurosecretory cells from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of 10% fetal bovine serum, 5% horse serum, and 100 units of penicillin / DMEM culture medium (dubecco'sminimallessentialmedium, DMEM) of 100 micrograms / ml of streptomycin, centrifuged at 1000 rpm for 10 minutes, discarded the supernatant, added 4 ml of the above-mentioned medium, pipet evenly, and sucked it into the culture bottle. Change medium every other day, 37°C, 5% CO 2 Cultivation and passage under conditions; after the above-mentioned cultured cell line was passed for 3 generations, it was induced to differentiate into neuron-like cells by using DMEM medium with 5 ng / ml NGF an...

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Abstract

The invention discloses application of total paeony glycosides to preparation of a medicine for preventing or treating microwave radiation induced nerve cell injury, a pharmaceutical composition and food. According to the application of total paeony glycosides to preparation of the medicine, the medicine is used for preventing or treating microwave radiation induced nerve cell injury. Therefore, total paeony glycosides can be used for preparing the medicine for preventing and treating microwave radiation induced nerve cell injury, further, the prepared medicine can be used for administration prevention for people or animals probably suffering from microwave radiation, or can be used for administration treatment for people or animals suffering from microwave radiation induced nerve cell injury so as to achieve the purposes of reducing nerve cell injury and repairing injured nerve cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of total glucosides of paeony in the preparation of medicines for preventing or treating nerve cell damage caused by microwave radiation, a pharmaceutical composition and food. Background technique [0002] The biological hazard of microwave radiation has attracted more and more attention from scholars at home and abroad. The brain is a sensitive target organ for microwave radiation. Studies have shown that microwave radiation can cause damage to the structure and function of nerve cells. Studies have found that the oxidative stress caused by the excessive generation and accumulation of ROS is one of the important mechanisms for the structural damage and dysfunction of nerve cells caused by microwave radiation. ROS is a chemically active oxygen-containing atom or atomic group. Its large-scale production and accumulation can cause peroxidation of unsaturated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/65A61K36/71A61P25/00A23L1/29
Inventor 彭瑞云赵黎王惠王丽峰王浩宇胡韶华陈俊俊郝延辉喻超
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA