Preparation method and applications of porous composite material with peroxidase activity

A porous composite material, peroxidase technology, applied in chemical instruments and methods, organic compound/hydride/coordination complex catalysts, oxidized water/sewage treatment, etc., can solve the problem of high cost, difficult purification, unstable It can improve the peroxidase activity, efficient catalytic degradation, and simple preparation method.

Inactive Publication Date: 2016-01-13
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, inconvenient storage and use, difficult purification and high cost, sensitivity of catalytic activity under environmental conditions, self-instability and poor recovery have restricted the application of enzymes in some fields.

Method used

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  • Preparation method and applications of porous composite material with peroxidase activity
  • Preparation method and applications of porous composite material with peroxidase activity

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Embodiment 1

[0020] A preparation method of a porous composite material with peroxidase activity, which uses an acid-free method to hydrothermally synthesize MIL-100(Fe), and obtains a porous composite material derived from MIL-100(Fe) by calcining MIL-100(Fe) materials, the steps are as follows:

[0021] 1) Preparation of MIL-100(Fe)

[0022] 2.5mmolFe(NO 3 ) 3 9H 2 O and 1.675 mmol of trimesic acid were dissolved in 12.5 mL of distilled water, ultrasonically dissolved, then transferred to a reaction kettle with a Teflon liner, reacted at 160 ° C for 12 h, centrifuged to separate the solid, and successively washed with water, N, N - After washing with dimethylformamide and ethanol, dry under vacuum at 100°C for 12 hours to obtain MIL-100(Fe);

[0023] 2) Preparation of MIL-100(Fe)-derived porous composites

[0024] Put the above-prepared MIL-100(Fe) in a muffle furnace, heat up to 350°C at a rate of 2°C / min in the air, calcinate at this temperature for 120min, and cool the obtained c...

Embodiment 2

[0030] A preparation method of a porous composite material with peroxidase activity, which uses an acid-free method to hydrothermally synthesize MIL-100(Fe), and obtains a porous composite material derived from MIL-100(Fe) by calcining MIL-100(Fe) materials, the steps are as follows:

[0031] 1) Preparation of MIL-100(Fe)

[0032] 2.5mmolFe(NO 3 ) 3 9H 2 O and 1.675mmol of trimesic acid were dissolved in 37.5mL of distilled water, ultrasonically dissolved, then transferred to a reaction kettle with a Teflon liner, reacted at 160°C for 12h, centrifuged to separate the solid, followed by water, N, N - After washing with dimethylformamide and ethanol, dry under vacuum at 100°C for 12 hours to obtain MIL-100(Fe);

[0033] 2) Preparation of MIL-100(Fe)-derived porous composites

[0034] The above-prepared MIL-100(Fe) was placed in a muffle furnace, heated to 600°C in argon at a rate of 5°C / min, calcined at this temperature for 60min, and the obtained composite material was coo...

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Abstract

The invention discloses a preparation method of a porous composite material with peroxidase activity. The preparation method comprises following steps: acid-free hydro-thermal synthesis is adopted to synthesize MIL-100(Fe); MIL-100(Fe) is subjected to calcining so as to obtain the MIL-100(Fe) derived porous composite material which can be used for catalytic degradation of methylene blue. The preparation method of the porous composite material based on MIL-100(Fe) is simple, and is convenient to realize; calcining of MIL-100(Fe) is capable of maintaining original material porosity, generating high density bio simulation active centers, and increasing peroxidase activity of the MIL-100(Fe) derived porous composite material greatly; so that high efficiency catalytic degradation of organic dye methylene blue can be realized with small catalyst amount and at a relatively high temperature.

Description

technical field [0001] The invention relates to the preparation of a porous composite material with peroxidase activity, in particular to a preparation method and application of a porous composite material with peroxidase activity. Background technique [0002] As a highly efficient biocatalyst, enzymes in nature can effectively participate in various reactions. Due to its high efficiency, high specificity and mild reaction conditions, enzyme-catalyzed reactions are widely used. However, inconvenient storage and use, difficult purification and high cost, sensitivity of catalytic activity under environmental conditions, self-instability and poor recovery have restricted the application of enzymes in some fields. Therefore, artificial enzymes have gradually become a hot spot in the field of materials research. Designing and synthesizing biomimetic enzymes with remarkable catalytic activity, good durability and substrate selectivity has become a huge challenge, which is also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/22C02F1/58C02F1/72
Inventor 黄艳凤刘民李颖张纪梅晏维语
Owner TIANJIN POLYTECHNIC UNIV
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