Preparation method and application of fluorescent probe

A fluorescent probe and fluorescent sensing technology, applied in the field of chemical sensing materials, achieves the effects of simple post-reaction processing, reduced detection cost, and mild reaction conditions

Inactive Publication Date: 2016-01-13
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these fluorescent molecular probes have different shortcomings, so it is necessary and urgent to design more probe molecules with good performance.

Method used

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  • Preparation method and application of fluorescent probe
  • Preparation method and application of fluorescent probe
  • Preparation method and application of fluorescent probe

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of preparation method of fluorescent probe, such as figure 1 In the synthetic route shown, 180 mg of carbohydrazide and 861 mg of hydroxyanthral are dissolved in 44 mL of ethanol / water = 10:1 mixed solution of 1 mL of glacial acetic acid, stirred and refluxed at a medium speed at 80 ° C for 12 hours, and the reaction solution After high-speed centrifugation, the precipitate was washed with anhydrous methanol. The yield is 99%, and it is named as the Schiff base fluorescent sensing material.

[0031] An application method of a fluorescent probe, the specific steps are as follows:

[0032] Step 1, the Schiff base fluorescent sensing material is prepared into C=1×10 with DMF -5 mol / L Schiff base fluorescent sensing material dispersion.

[0033] Step 2. Prepare Hg 2+ A solution having a molar ratio of 0.5, 1, 2, 5, 10, 20, 30, 40, 50 to the Schiff base fluorescent sensing material; mixing the dispersion prepared in step 1 with the solution respectively to obtain ...

Embodiment 2

[0040] Preparation of Schiff base fluorescent sensing material: Dissolve 360mg of carbohydrazide and 1.812g of hydroxyanthral in 50mL mixed solution of ethanol / water=8:1 dispersed with 5mL of glacial acetic acid, stir and reflux at a medium speed at 90°C After 10 hours, the reaction solution was centrifuged at high speed, and the precipitate was washed with anhydrous methanol. Yield 96%.

[0041] An application method of a fluorescent probe, the specific steps are as follows:

[0042] Step 1, the Schiff base fluorescent sensing material is prepared into C=1×10 with DMF -5 mol / L Schiff base fluorescent sensing material dispersion.

[0043] Step 2. Prepare Hg 2+ A solution having a molar ratio of 0.5, 1, 2, 5, 10, 20, 30, 40, 50 to the Schiff base fluorescent sensing material; mixing the dispersion prepared in step 1 with the solution respectively to obtain a mixed solution; Measure the fluorescence intensity value of the mixed solution. Measure the change of the fluorescen...

Embodiment 3

[0051] Preparation of Schiff base fluorescent sensing material: Dissolve 120mg of carbohydrazide and 618mg of hydroxyanthral in 40mL mixed solution of ethanol / water=9:1, stir and reflux at medium speed at 95°C for 12 hours, and the reaction solution is passed through high speed Centrifuge, and wash the precipitate with anhydrous methanol. Yield 94%.

[0052] An application method of a fluorescent probe, the specific steps are as follows:

[0053] Step 1, the Schiff base fluorescent sensing material is prepared into C=1×10 with DMF -5 mol / L Schiff base fluorescent sensing material dispersion.

[0054] Step 2. Prepare Hg 2+ A solution having a molar ratio of 0.5, 1, 2, 5, 10, 20, 30, 40, 50 to the Schiff base fluorescent sensing material; mixing the dispersion prepared in step 1 with the solution respectively to obtain a mixed solution; Measure the fluorescence intensity value of the mixed solution. Measure the change of the fluorescence intensity value of the mixed solutio...

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Abstract

The invention relates to a preparation method and application of a fluorescent probe and belongs to the field of chemical sensing materials. The fluorescent probe is applied in the field of mercury ion detection. The preparation method comprises a step of dissolving carbohydrazide and hydroxylanthraldehyde in a mixture solution composed of ethanol and water and adding an acidic solution dropwisely to prepare the fluorescent probe, which is named Schiff base fluorescent sensing material. The Schiff base fluorescent sensing material then is applied in the mercury ion detection. The fluorescent probe, which is simple in structure and is easy to prepare, is very high in selectivity and sensitivity to Hg<2+> ion in a N,N-dimethylformamide solution. The fluorescent probe has fluorescence enhancement on the Hg<2+> ion and is 1*10<-5> mol / L in lowest detectable limit of the Hg<2+> ion concentration. The compound has high-sensitivity and high-selectivity fluorescent detection to the Hg<2+> ion without interferences caused by other ions, such as Ag<+>, Zn<2+>, Co<2+>, Ca<2+>, Cu<2+>, Ba<2+>, Pb<2+>, Mg<2+>, Ni<2+>, Cd<2+> and the like, so that the fluorescent probe can be applied widely in a Hg<2+> ion sensor with fluorescent channel detection.

Description

technical field [0001] The invention relates to a preparation method and application of a fluorescent probe, belonging to the field of chemical sensing materials. Background technique [0002] Mercury is a chemical substance with severe physiological toxicity. Because of its persistence, easy migration and high bioaccumulation, it has become one of the environmental pollutants that attract global attention. Mercury in the environment can be highly enriched in biological tissues through the food chain, thus causing great harm to humans and nature. Therefore, how to effectively detect mercury ions is of great significance to biological science, environmental science and medicine. [0003] So far, the detection methods for mercury ion standards mainly include cold atomic absorption spectrometry and disulfide colorimetric method. However, these methods generally have problems such as insufficient detection speed, high detection price, complicated operation, and large interfere...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 单博刘云飞束庆海金韶华陈树森
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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