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Fixable oxycarbide and microorganism capable of performing fermentation reaction and preparation method and application thereof

一种氧化物、固定碳的技术，应用在基于微生物的方法、生物化学设备和方法、微生物等方向，能够解决有机化合物产率不佳、成本及资源浪费、碳源损失等问题

Inactive Publication Date: 2016-01-20
GREEN CELLULOSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the above-mentioned process of converting pyruvate into acetyl-CoA will be accompanied by the release of carbon oxides such as carbon dioxide, resulting in unnecessary carbon loss from raw materials to products.
It is known that if a natural wild-type strain is used to carry out the ABE fermentation process, the carbon conversion rate of converting raw materials into products is only about 67%, resulting in poor yields of organic compounds, resulting in unnecessary costs and waste of resources

Method used

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  • Fixable oxycarbide and microorganism capable of performing fermentation reaction and preparation method and application thereof
  • Fixable oxycarbide and microorganism capable of performing fermentation reaction and preparation method and application thereof
  • Fixable oxycarbide and microorganism capable of performing fermentation reaction and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1: the preparation of fusion strain

[0072] (1-1) Selection of parental strains

[0073] Clostridium ljungdahlii strain BCRC17797 capable of fixing carbon dioxide and Clostridium tyrobutyricum strain BCRC14535 capable of fermentation to produce organic acids were selected as parental strains.

[0074] (1-2) Preparation of protoplasts

[0075] Mix glucose and mRCM medium to provide a mixed medium with a glucose concentration of 10 g / L, and deoxygenate the mixed medium, and then inject 40 ml of deoxygenated centrifuge tubes into two deoxygenated centrifuge tubes mixed media. Next, inoculate the two parental strains selected in (1-1) into the mixed culture medium of the centrifuge tube, and cultivate them in an anaerobic incubator at 37°C until the Dassostella grows to OD 600 (the absorbance value when the wavelength is 600 nanometers) is about 0.8, Clostridium tyrobutyricum grows to OD 600 About 1.5 (ie, both parental strains grew to mid-log phase). The b...

Embodiment 2

[0084] Example 2: Test the physiological properties of Clostridium tyrobutyricum strain ITRI02001 and Clostridium tyrobutyricum strain BCRC14535

[0085] Glucose was mixed with the mRCM medium to provide a mixed medium with a glucose concentration of 10 g / L, and the mixed medium was deoxygenated for subsequent use.

[0086] Inject 40 ml of the aforementioned deoxygenated mixed medium into two 50 ml centrifuge tubes. Next, the Clostridium tyrobutyricum strain BCRC14535 and the Clostridium tyrobutyricum strain ITRI02001 were respectively inoculated in the aforementioned mixed medium, and cultured in an anaerobic incubator at 37°C. When the strains grow to the mid-logarithmic phase (that is, the OD of Clostridium tyrobutyricum strain BCRC14535 and Clostridium tyrobutyricum strain ITRI02001 600 Respectively about 1.5 o'clock), the bacterium liquid of this two bacterial strains is with the volume ratio of 1: 200 (bacteria liquid: mRCM medium), is diluted in the two airtight bottle...

Embodiment 3

[0089] Example 3: Testing the effectiveness of Clostridium tyrobutyricum strain ITRI02001 in producing organic compounds

[0090] (3-1) Comparison of carbon conversion rate between Clostridium tyrobutyricum strain BCRC14535 and ITRI02001

[0091] Glucose and mRCM medium were mixed to provide a mixed medium with a glucose concentration of 10 g / L and an acetic acid concentration of 4 g / L, and the mixed medium was deoxygenated for subsequent use.

[0092] Inject 40 ml of the aforementioned deoxygenated mixed medium into two 50 ml centrifuge tubes. Next, the Clostridium tyrobutyricum strain BCRC14535 and the Clostridium tyrobutyricum strain ITRI02001 were respectively inoculated in the aforementioned mixed medium, and cultured in an anaerobic incubator at 37° C. for fermentation reaction for 48 hours. Next, the contents of glucose, acetic acid and butyric acid in the aforementioned medium were analyzed with an Agilent 1100 series high-performance liquid chromatography coupled wit...

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Abstract

A fixable oxycarbide and a microorganism capable of performing fermentation reaction and a preparation method and application thereof are characterized in that the preparation method includes: (a) providing a first parental generation bacterial strain capable of fixing the oxycarbide; (b) providing a second parental generation bacterial strain capable of performing the fermentation reaction; (c) respectively preparing the first parental generation bacterial strain and the second parental generation bacterial strain into a first protoplast and a second protoplast; (d) fusing the first protoplast and the second protoplast so as to provide a fused bacterial strain; and (e) cultivating the fused bacterial strain so as to obtain an object bacterial strain which can be used for producing organic compounds (such as organic acids and alcohols). A specific sample of the object bacterial strain is Colstridium tyrobutyricum strian ITRI02001 and is stored in Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). The storage number is DSM 32077.

Description

technical field [0001] The present invention relates to a microorganism capable of fixing carbon oxides and performing fermentation reactions and its preparation method and application, especially the application of the microorganism in the production of organic compounds such as organic acids and alcohols. A specific aspect of the microorganism is the Clostridium tyrobutyricum strain ITRI02001, which is deposited in the German National Culture Collection (DSMZ) with the deposit number DSM32077. Background technique [0002] Since the beginning of the 20th century, bacteria, yeasts, molds and other microorganisms have been widely used in industry to carry out fermentation reactions to convert biomass raw materials into more economical organic compounds, such as organic acids and alcohols. Among them, the acetone-butanol-ethanol (ABE) fermentation procedure is most widely used among the microbial fermentation methods for producing organic compounds. In the ABE fermentation p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/03C12N1/21C12P7/16C12P7/04C12P7/06C12P7/56C12P7/52C12P7/54C12R1/145
CPCC12N15/03C12P7/04C12P7/065C12P7/16C12P7/54C12P7/56Y02E50/10C12N1/20C12P7/52C12R2001/145C12N1/205
Inventor 童迁祥陈昌杰徐勤祯周宜德
Owner GREEN CELLULOSITY
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