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Method for preserving nucleic acid on solid surface, and preservation solution thereof

A technology of solid surface and preservation solution, which is applied in the field of temperature preservation of nucleic acid, can solve the problem of RNA that can only be preserved in a liquid state at room temperature for several days or weeks, and achieve the effect of saving nucleic acid extraction time and prolonging the extraction time

Inactive Publication Date: 2016-01-20
SHANGHAI ADICON CLINICAL LAB LNC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most commercially available RNA preservation products but only store RNA in liquid state at room temperature for days or weeks

Method used

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  • Method for preserving nucleic acid on solid surface, and preservation solution thereof

Examples

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Effect test

example 1

[0043] First add proper amount of clean water, then add Tris-hydrochloric acid (20mmol / lTris-HCl, pH8.3, 20℃), potassium chloride solution (20mmol / l), and magnesium chloride solution (1mmol / l) as buffers . After that, Tween 20 (0.01% by mass) and TritonX-100 (0.01% by mass) were added as non-ionic surfactants. Next, add surface active peptide (0.01% by mass), and bovine serum albumin (BSA, 1 mg / ml). Thus, a nucleic acid extraction preservation solution according to an embodiment of the present invention is formed.

example 2

[0045] First add proper amount of clean water, and then add Tris-hydrochloric acid (400mmol / lTris-HCl, pH8.3, 20℃), potassium chloride solution (50mmol / l), and magnesium chloride solution (3mmol / l) as buffers . After that, Tween 20 (1% by mass) and TritonX-100 (1% by mass) were added as non-ionic surfactants. Next, add surface-active peptide (1% by mass) and bovine serum albumin (BSA, 3 mg / ml). Thus, a nucleic acid extraction preservation solution according to an embodiment of the present invention is formed.

example 3

[0047] First add an appropriate amount of clean water, then add Tris-hydrochloric acid (200mmol / lTris-HCl, pH8.3, 20℃), potassium chloride solution (25mmol / l), and magnesium chloride solution (1.5mmol / l) as buffers Agent. After that, Tween 20 (0.5% by mass) and TritonX-100 (0.5% by mass) were added as non-ionic surfactants. Next, add surface-active peptide (0.5% by mass) and bovine serum albumin (BSA, 1.5 mg / ml). Thus, a nucleic acid extraction preservation solution according to an embodiment of the present invention is formed.

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Abstract

The present invention provides a method for preserving nucleic acid on the solid surface, and a preservation solution thereof, and relates to a nucleic acid extraction and preservation solution, which comprises a buffer, a non-ionic surfactant, a biological surfactant and bovine serum albumin. According to the present invention, with the preservation solution, cells and other samples are placed into the nucleic acid extraction and preservation solution to make DNA be stably preserved in the nucleic acid extraction and preservation solution so as to save the nucleic acid extraction time of the laboratory; according to the existing detection kit, the sample is generally preserved in the nucleic acid preservation solution, the extraction is performed by using other extraction methods after the sample returns to the lab, the whole process exceeds 1 h, and if the number of the sample is more, the extraction is substantially prolonged, such that the extraction rate determines more than or equal to half of the detection time of the sample; with the technical scheme of the present invention, the cells and other samples can be placed into the nucleic acid extraction and preservation solution to make DNA be stably preserved in the nucleic acid extraction and preservation solution so as to save the nucleic acid extraction time of the laboratory.

Description

Technical field [0001] The present invention relates to the temperature preservation of nucleic acid, in particular to a method for storing nucleic acid on a solid surface and its preservation solution. Background technique [0002] The development of microfluidic technology capable of extracting nucleic acids from cell lysates and other sources has facilitated the rapid analysis of nucleic acids in biological samples. Rapid extraction methods can be combined with amplification techniques such as polymerase chain reaction (PCR) to extract useful amounts of nucleic acids from trace samples of blood, tissue, cultured cells, or other biological materials. [0003] However, ribonucleic acid (RNA) is one of the most difficult biological molecules to preserve. Therefore, the extraction and preservation of RNA from biological samples is a difficult point. The conditions for degrading RNA include but not limited to the RNA used for extraction or collection, pH value, temperature, and mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 陈坚黎飒
Owner SHANGHAI ADICON CLINICAL LAB LNC
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