RT-PCR primers for detecting mink astroviruses and detection method using the same
A RT-PCR and astrovirus technology, which is applied in the field of RT-PCR primers and its detection for the detection of mink astrovirus, can solve the problems of lack of literature related to mink astrovirus, and achieve high sensitivity, low cost and suitable length Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0029] The mink astrovirus-positive disease materials used in the examples of the present invention; mink parvovirus; mink distemper virus; porcine epidemic diarrhea virus are all preserved by the Key Laboratory of Preventive Veterinary Medicine of Qingdao Agricultural University.
[0030] Step 1, design and synthesis of primers: compare the nucleotide sequence ( figure 1 ), the relatively conserved region of the selected gene. Utilize Primeer5.0 software, take mink astrovirus (NC_004579.1) ORF1b gene sequence as a reference, design the specific primer that detects mink astrovirus ( figure 2 ,Table 1). The primers were synthesized by Beijing Saibaisheng Gene Technology Co., Ltd., diluted with DEPC water to 10 pmol / ul, and stored at -20 degrees Celsius for later use.
[0031] Table 1 Primer Sequence
[0032]
[0033] Note: Position in the whole genome of Minkastrovirus strain (NC_004579.1)
[0034] Step 2: Use TaKaRa's RNAisoPlus reagent to extract total RNA in the samp...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com