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A pretreatment method for paraffin section for tissue biopsy

A paraffin section and tissue biopsy technology, which is applied in the field of biopathological tissue detection, can solve the problems of long tissue biopsy process cycle, unfavorable control costs, and large consumption of dehydrating agent, and achieves optimization of tissue biopsy process, shortening biopsy cycle, and easy control of the process. Effect

Active Publication Date: 2018-11-20
GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paraffin section method includes such steps as material collection, fixation, washing and dehydration, clearing, paraffin immersion, embedding, sectioning and sticking, dewaxing, staining, dehydration, clearing, and mounting, among which the two steps of dehydration and clearing are the most time-consuming, generally It takes a few days for the tissue to be fixed and sealed to make a slide specimen, which makes the tissue biopsy process cycle long and inefficient
[0003] Chinese patent application CN201010282261.9 discloses a biological tissue dehydrator and its application. The dehydration method of the dehydrator is to fix the biopathological tissue in the sample tank, and continuously feed the dehydrating agent whose concentration changes continuously from low to high. Dehydration, the dehydration speed of the tissue is accelerated by continuously feeding the dehydrating agent, but the dehydrating agent is consumed too much during the dehydration process, resulting in waste, which is not conducive to cost control

Method used

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  • A pretreatment method for paraffin section for tissue biopsy
  • A pretreatment method for paraffin section for tissue biopsy
  • A pretreatment method for paraffin section for tissue biopsy

Examples

Experimental program
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Effect test

Embodiment 1

[0029] After fixing the tissue with a thickness of less than 3mm and an area of ​​less than 15mm×15mm with 10% neutral buffered formalin for 6 hours, the tissue was dehydrated, transparent and wax-dipped under normal temperature and pressure conditions. The steps are:

[0030] 1) Immerse the fixed tissue in 95% ethanol for 5 minutes, and use a stirrer to stir the dehydrated solution at 800 rpm;

[0031] 2) After immersion in 95% ethanol for 5 minutes, the tissue was immersed in 100% ethanol for 5 minutes, and at the same time, the dehydration solution was stirred at 800 rpm with a stirrer;

[0032] 3) After immersion in 100% ethanol for 5 minutes, the tissue was immersed in 100% ethanol for 7 minutes, and the dehydration solution was stirred at 800 rpm with a stirrer;

[0033] 4) After immersion in 100% ethanol for 7 minutes, the tissue was immersed in 100% ethanol for 16 minutes, and at the same time, the dehydration solution was stirred at 800 rpm with a stirrer;

[0034] 5...

Embodiment 2

[0043] After fixing the tissue with a thickness of less than 3mm and an area of ​​less than 15mm×15mm with 10% neutral buffered formalin for 6 hours, the tissue was dehydrated, transparent and wax dipped under the condition of heating and pressure. The steps are:

[0044] 1) Under the temperature of 65 degrees and the pressure of 35KPa, the fixed tissue was immersed in 95% ethanol for 5 minutes, and the dehydration solution was stirred at 800 rpm with a stirrer;

[0045] 2) Under the temperature of 65 degrees and the pressure of 35KPa, the tissue after immersion in 95% ethanol for 5 minutes was immersed in 100% ethanol for 5 minutes, and the dehydration solution was stirred at 800 rpm with a stirrer;

[0046] 3) Under the temperature of 65 degrees and the pressure of 35KPa, the tissue after immersion in 100% ethanol for 5 minutes was immersed in 100% ethanol for 7 minutes, and the dehydration solution was stirred at 800 rpm with a stirrer;

[0047] 4) Under the temperature of ...

Embodiment 3

[0057] Tissues with a thickness less than 3mm and an area less than 15mm×15mm were fixed with 10% neutral buffered formalin for 6 hours, and then dehydrated, transparentized and soaked in wax under conditions of heat and pressure, such as image 3 As shown, the steps are:

[0058] 1) At a temperature of 60°C and a pressure of 35KPa, soak the fixed tissue with 10% neutral buffered formalin for 11 minutes, and at the same time use a stirrer to stir the dehydration solution at 800 rpm;

[0059] 2) At a temperature of 65 degrees and a pressure of 35KPa, the tissue was impregnated with 10% neutral buffered formalin for 11 minutes and impregnated with 90% formaldehyde ethanol for 16 minutes. stirring;

[0060] 3) At a temperature of 65 degrees and a pressure of 35KPa, the tissue impregnated with 90% formaldehyde and ethanol is impregnated with 95% ethanol for 5 minutes, and at the same time, use a stirrer to stir the dehydration solution at 800 rpm;

[0061] 4) At a temperature of...

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Abstract

The invention discloses a pretreatment method of a paraffin section for tissue biopsy, and belongs to the field of biological pathology tissue detection. The pretreatment method includes the step that 10% neutral buffered formalin fixation, 90% formaldehyde and ethanol fixation, ethanol gradient dehydration, xylene transparentizing and paraffin impregnating are sequentially performed on fixed tissue under corresponding temperature, pressure, time and rotating speed conditions. The pretreatment method has the advantages that through the optimum combination of reagents and the optimization of dehydration, transparentizing and impregnating conditions, the pretreatment efficiency of the paraffin section is improved, the dehydration, transparentizing and impregnating time of tissue biopsy is shortened to about two hours from about ten hours needed in a traditional method, the biopsy period is shortened, and the usage ratio of equipment is greatly increased.

Description

technical field [0001] The invention belongs to the field of biopathological tissue detection, in particular to a pre-processing method for paraffin sections used for tissue biopsy. Background technique [0002] Paraffin section is not only used to observe the morphological structure of normal cells, but also the main method used by pathology and forensics to study, observe and judge the morphological changes of cells. The paraffin sectioning method includes the steps of sampling, fixing, washing and dehydration, clearing, dipping in wax, embedding, sectioning and sticking, dewaxing, staining, dehydration, clearing, and sealing. Among them, the two steps of dehydration and clearing are the most time-consuming and generally It takes several days for the tissue from the sample to be fixed to the cover slip to make a slide specimen, which makes the tissue biopsy process long and inefficient. [0003] Chinese patent application CN201010282261.9 discloses a biological tissue deh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 刘柱新钟学军陈嘉昌柳俊张瑶陈肖燕
Owner GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD