Method for measuring content of multiple active ingredients in xiaoshuan tongluo tablets

Abstract

The invention belongs to the field of traditional Chinese medicine and particularly relates to a method for measuring the content of main active ingredients in xiaoshuan tongluo tablets. According to the measurement method, the content of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in the xiaoshuan tongluo tablets can be measured simultaneously with an HPLC (high performance liquid chromatography) method, a reference substance mixed solution I and a test solution I are injected into a liquid chromatograph respectively according to chromatographic conditions, the injection volume of the reference substance mixed solution I is 10 mu L, the injection volume of the test solution I is 5 mu L, the chromatographic peak area is recorded, and the content is calculated with an external standard method. Meanwhile, according to the measurement method, the content of astragaloside is measured with an ELSD (evaporative light-scattering detector) method, a reference substance mixed solution II and a test solution II are injected into the liquid chromatograph respectively according to chromatographic conditions, the injection volumes of the reference substance mixed solution II are 5 mu L and 10 mu L respectively, the injection volume of the test solution II is 10-20 mu L, the chromatographic peak area is recorded, and the content is calculated according to a two-point external standard method logarithmic equation.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a content determination method of main active ingredients in Xiaoshuan Tongluo Tablets. Background technique [0002] Xiaoshuan Tongluo Tablets are composed of Chuanxiong, Salvia, Astragalus, Alisma, Panax notoginseng, Sophora japonica, Curcuma, Guizhi, Woody, Borneol and Hawthorn. It has the functions of promoting blood circulation and removing blood stasis, warming meridians and dredging collaterals. It is used for hyperlipidemia, mental sluggishness caused by cerebral thrombosis, hard tongue, slow speech, slurred pronunciation, cold hands and feet, pain in movement and other diseases. The preparation is included in the Chinese Pharmacopoeia 2010 edition one. Studies have shown that it has the characteristics of improving cardiovascular function and removing blood stasis without harming the body, so it is widely used in clinical treatment. [0003] The...

AI Technical Summary

Problems solved by technology

However, this poses a challenge to the efficiency of quality control. If fast thin-layer scanning is used for quantification, the accuracy will be poor. If chromatography is used for determination, it will be very time-consuming and significantly increase the cost of quality control. Simultaneous determination of several components in the same chromatographic system is an ideal quality control method

[0004] In the first part of the Chinese Pharmacopoeia 2010 edition, the quality control index for Xiaoshuan Tongluo Tablets is single, only for the detection of ginsenoside Rg1 in Panax notoginseng in this preparation