Fluorescent quantitative PCR detecting kit for hepatitis E virus and using method of fluorescent quantitative PCR detecting kit

A technology of hepatitis E virus and detection kit, applied in the field of medical detection, can solve problems such as poor accuracy, and achieve the effects of improving detection sensitivity, fast operation and good adsorption effect

Inactive Publication Date: 2016-02-03
SANSURE BIOTECH INC
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Problems solved by technology

[0006] The purpose of the present invention is to provide a hepatitis E virus fluorescent quantitative PCR detection kit and...

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  • Fluorescent quantitative PCR detecting kit for hepatitis E virus and using method of fluorescent quantitative PCR detecting kit
  • Fluorescent quantitative PCR detecting kit for hepatitis E virus and using method of fluorescent quantitative PCR detecting kit
  • Fluorescent quantitative PCR detecting kit for hepatitis E virus and using method of fluorescent quantitative PCR detecting kit

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Embodiment Construction

[0050] The hepatitis E virus fluorescent quantitative PCR detection kit and its use method provided by the embodiments of the present invention solve the problem of poor accuracy of existing HEV detection methods.

[0051] In order to enable those skilled in the art to better understand the technical solutions in the embodiments of the present invention, and to make the above-mentioned purposes, features and advantages of the embodiments of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention are described below in conjunction with the accompanying drawings The program is described in further detail.

[0052] The hepatitis E virus fluorescent quantitative PCR detection kit provided by the embodiments of the present invention includes a PCR reaction solution, and the PCR reaction solution includes PCR buffer, deoxyribonucleoside triphosphate, DNA polymerase, and is used to amplify target polynuclear nucleotid...

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Abstract

The invention provides a fluorescent quantitative PCR detecting kit for a hepatitis E virus. The detecting kit comprises a PCR reaction solution, wherein the PCR reaction solution comprises a PCR buffering solution, deoxyribonucleoside triphosphate, a DNA polymerase, an upstream primer, a downstream primer and a probe, wherein the upstream primmer and the downstream primer are used for amplifying target polynucleotide, and the probe is used for detecting the target polynucleotide. The fluorescent quantitative PCR detecting kit for the hepatitis E virus is favorable in specificity, high in operation speed, simple and convenient in method and wide in detection range, and meanwhile an interior label additionally arranged in the reaction system can be used for effectively avoiding that the detected result is false negative. When the fluorescent quantitative PCR detecting kit for the hepatitis E virus is used for extracting RNA, a magnetic bead method which is good in adsorption effect and easy to purify is used, and the method can be used for effectively removing PCR inhibitors in complex samples, so that RNA with high purity and high yield is obtained, and detecting sensitivity, accuracy and stability are greatly improved.

Description

technical field [0001] The invention relates to the technical field of medical detection, and more specifically relates to a hepatitis E virus fluorescent quantitative PCR detection kit and a use method thereof. Background technique [0002] Hepatitis E virus (Hepatitis E virus, HEV) is the pathogen of hepatitis E (hereinafter referred to as "hepatitis E"), which is mainly transmitted through the fecal-oral route, and can cause sporadic, outbreak and epidemic in the crowd. HEV belongs to the hepatitis E family in virological classification, and mammalian HEV can be divided into at least 4 kinds of genotypes, namely genotype 1, 2, 3 and 4 types of HEV (hereinafter referred to as HEV-1, HEV-2, HEV-3 and HEV-4). Through the research, it is found that each genotype has obvious geographical distribution characteristics: genotype 1 is mainly distributed in Asia and North Africa; genotype 2 is mainly distributed in Mexico and some West African countries; genotype 3 is distributed ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/707C12Q1/6806C12Q1/686C12Q2600/166C12Q2545/101C12Q2545/113C12Q2527/125C12Q2563/143C12Q2563/149
Inventor 戴立忠丁海周雷刘佳邓中平
Owner SANSURE BIOTECH INC
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