Specificity molecular marker for identifying Taicang Xinmao taros and screening method thereof
A technology of Taro chinensis and molecular markers, which is applied to biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., and can solve the specificity and specificity identification molecules of Taro sativa that have not been developed yet. Marking and other issues
Inactive Publication Date: 2016-03-02
苏州市种子管理站 +1
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However, at present, the identification of Taurus chinensis is mainly based on its morphological characteristics, and molecular markers that can identify the specificity and specificity of Taurus chinensis have not been developed yet.
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[0025] The methods used in the following examples are conventional methods unless otherwise specified. Primers were synthesized by Nanjing Ruizhen Biotechnology Co., Ltd.; DNA extraction kit was purchased from Beijing Quanshijin Company, Taq enzyme and DL2000DNAmarker were purchased from Guangzhou Dongsheng Biotechnology Co., Ltd. pMD19-TVector and Escherichia coli DH5α competent cells were purchased from Takara The company's products; the TissueLyserLT tissue disruptor is a product of QIAGEN in Germany; the nucleic acid and protein detector is a product of eppendorf in the United States; the PCR instrument is a BioMetraT1 type. Embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0026] , Plant genome total DNA extraction
[0027] A total of 9 taro varieties (Table 1) commonly cultivated in Taicang Xinmao taro and Jiangsu and Fujian regions were selected, and their genomic DNA was extracted respectively. Spe...
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The invention discloses a specificity molecular marker for identifying Taicang Xinmao taros and a screening method thereof. The nucleotide sequence of a specificity fragment obtained by amplification in the Taicang Xinmao taros is as shown in SEQ ID NO: 16. The screening method comprises the steps of first screening a chloroplast fragment primer capable of amplifying different sequences in taros with numerous cormels and taros of solitary, a locus specificity primer is designed according to the different sequences, and thus the taros with numerous cormels and the taros of solitary are distinguished. An SSR primer capable of amplifying a specificity strip in the Taicang Xinmao tarosis is screened in the taros with numerous cormels, a sequence specificity primer is designed according to the specificity strip, finally the Taicang Xinmao taros are distinguished from other taro varieties, the specificity identification of the Taicang Xinmao taros is achieved, and forceful technical support is provided for taro variety identification and genetic resource conservation.
Description
technical field [0001] The present invention belongs to the field of molecular marker identification, and in particular relates to a specific molecular marker for identifying Taicang new hairy taro and a screening method thereof. Background technique [0002] taro( Colocasia esculenta (Linnaeus) Schott) is a perennial herbaceous plant of the genus Colocasia of the family Araceae. It is native to tropical regions such as my country, India, and the Malay Peninsula, and is widely cultivated in the world. The tubers, flowers, leaves and other organs of taro can be used for food or medicine, and some countries or regions even use it as a staple food. Because taro has a long history of cultivation, its germplasm resources are very rich. The chromosome base of Taro is X=14, and there are diploid and triploid. According to the corm tillering, number of chromosomes and geographical distribution of taro, researchers in my country divide taro into four categories, namely taro, tar...
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IPC IPC(8): C12N15/11C12Q1/68
Inventor 沈雪林张艳梅戴华军孙小芹曹敏旭杭悦宇
Owner 苏州市种子管理站