A kind of natural hirudin circulation extraction device and extraction method
A natural hirudin, recycling extraction technology, applied in the preparation method of peptides, from leech inhibitors, chemical instruments and methods, etc., can solve the problems of leech resource destruction, achieve high extraction rate, promote reproduction and expansion, and simple process Effect
Active Publication Date: 2018-10-16
深圳蛭爱天下文化传播有限公司
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Problems solved by technology
[0003] The current method of extracting natural hirudin is to obtain hirudin by harming leeches, which also causes damage to leech resources.
Method used
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Embodiment 1
[0032] ⑴Material preparation: select fresh pig blood and heat it to 100°C and keep it for 5 minutes to sterilize. The pulp is beaten in a cooking machine, and the viscosity is adjusted to the concentration of fresh animal blood. The plasma is used as feed for leeches and as a carrier for extracting hirudin.
[0033] (2) Acclimatization and feeding: Pour the processed plasma into the liquid collection tank, and make sheep casings with a diameter of 5 cm and a length of 100 cm, connect the discharge pipe and the feed pipe, place them in the tray, and run a 24-volt DC water pump to make Plasma reciprocates in the sheep casings, and coats the processed plasma on the outer wall of the sheep casings, covers the tray with 80-mesh nylon gauze, and smears an appropriate amount of plasma to lure the domesticated hungry leeches to eat; During the feeding period, it is necessary to maintain sufficient internal pressure of the blood plasma to fill the feeding cannula; the population densit...
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Abstract
A hirudin extraction device includes a liquid collection tank, a water pump, a tray and a feeding casing tube, the tray is suspended in a leech breeding pool, the liquid collection tank is arranged on one side of the tray, the bottom of the liquid collection tank is provided with the water pump, the top of the water pump is communicated with a material discharge tube, the material discharge tube is fixedly arranged on the outer wall of the liquid collection tank, and extends outwardly to above the tray, one side of the outer wall of the liquid collection tank is fixed provided with a feed tube, wherein the side of the outer wall of the liquid collection tank is opposite to the material discharge tube; the tray is fixed below the feed tube and the material discharge tube by a fixation rack, and the tray is used for supporting the feeding casing tube in U-shaped communication with the feed tube and the material discharge tube. The process is simple, extraction rate is high, the extraction process is hirudo natural feeding process, a feeding liquid is not added with any chemical ingredients, may not harm hirudo, and can be used for unlimited number of times in a hirudo life cycle, and the extraction is performed once every two weeks. Biological resources are fully used, meanwhile hirudo population reproduction and expansion can be protected and promoted, and the method is an ecological, friendly and harmonious extraction method.
Description
technical field [0001] The invention relates to the technical field of natural hirudin extraction from living leeches, in particular to a natural hirudin circulation extraction device and extraction method. Background technique [0002] Natural hirudin (hirudin) is extracted from leeches and their saliva. Natural hirudin consists of 65-66 amino acids to form a small molecular protein with a molecular weight of 7000 Daltons. Natural hirudin can irreversibly combine with thrombin to inactivate thrombin. It is the most powerful natural thrombin inhibitor found so far. In addition, it has a strong antithrombotic effect, and has inhibitory effects on various experimental thrombus models. Natural hirudin has preventive and therapeutic effects on various thrombotic diseases such as venous thrombosis, disseminated intravascular coagulation, cerebral coagulation, thrombophlebitis and coronary artery thrombosis, especially the treatment of venous thrombosis and disseminated intravas...
Claims
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IPC IPC(8): C07K14/815C07K1/14
CPCC07K14/815
Inventor 吴铁明张海峰
Owner 深圳蛭爱天下文化传播有限公司