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Long QT syndrome (LQTs)-related miRNA (microribonucleic acid) and application thereof

A technology for QT syndrome, mir-3619, applied in DNA/RNA fragments, recombinant DNA technology, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2016-03-09
NINGBO MEDICAL CENT LIHUILI HOSPITACL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, there is no drug for the treatment of long QT syndrome that promotes hERG gene function

Method used

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  • Long QT syndrome (LQTs)-related miRNA (microribonucleic acid) and application thereof
  • Long QT syndrome (LQTs)-related miRNA (microribonucleic acid) and application thereof
  • Long QT syndrome (LQTs)-related miRNA (microribonucleic acid) and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] Materials and Methods

[0009] 1.1 Experimental instruments and reagents

[0010] 1.1.1 Main experimental instruments

[0011] Table 1 Names and manufacturers of experimental instruments

[0012]

[0013]

[0014] 1.1.2 Experimental drugs and reagents

[0015] Table 2 Names and manufacturers of experimental drugs

[0016]

[0017]

[0018] 1.1.3 Experimental method

[0019] real-time fluorescent quantitative PCR

[0020] Total RNA was extracted from transfected U2OS cells with Trizol reagent. The RNA was subsequently treated with sterile RNase-free, and the level of hERG gene mRNA was assessed using Taqman probes and detected by qRT-PCR. Primers and probes are from ABI company cat.#4331182 (number: Hs04234270-g1). Reverse transcription conditions were set as an initial step at 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and a hold at 60°C for 1 min. Real-time PCR reactions were performed in triplicate independently. The results were anal...

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Abstract

The invention discloses a molecular marker capable of prognosing long QT syndrome (LQTs) attack risks and diagnosing the LQTs, namely miRNA (microribonucleic acid)-3619, and an application of antisense oligonucleotides AMO-3619 of miRNA-3619 in preparation of medicines for treating the LQTs, mainly an application of the medicines in promotion of human ether-a-Go-Go related gene (hERG) functions. The molecular marker is characterized in that the medicine composition contains AMO-3619.

Description

technical field [0001] The present invention relates to the discovery of a miRNA that can predict the risk and diagnosis of long QT syndrome, that is, miR-3619, and the application of its antisense oligonucleotide AMO-3619 in the preparation of drugs for the treatment of long QT syndrome. Specifically, the present invention relates to the application of AMO-3619 in promoting the function of hERG gene in the preparation of drugs for treating long QT syndrome. Background technique [0002] Long QT syndrome (LongQTSyndrome, LQTs) refers to the prolonged QT interval and abnormal T wave on the electrocardiogram, prone to ventricular arrhythmia, especially Torsade de pointes (TdP), syncope and sudden death a group of syndromes. According to the etiology, it can be divided into congenital long QT syndrome (cLQTs) and acquired long QT syndrome (aLQTs). cLQTs are a major cause of episodic syncope and sudden unexpected death in children and young adults. A variety of channel gene m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/68A61K48/00A61K31/7105A61P9/06
Inventor 郑梅霞廉姜芳周建庆杨曦黄晓燕庄凯丽国建毛海燕巴艳娜方培亮
Owner NINGBO MEDICAL CENT LIHUILI HOSPITACL
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