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Serum miRNA markers suitable for diagnosis of ossification of posterior longitudinal ligament and application thereof

A technology of posterior longitudinal ligament and diagnostic reagent, which is applied in the field of medical biological detection to achieve the effect of convenient and easy early screening and improve clinical treatment effect

Active Publication Date: 2016-03-16
SHANGHAI YUHUA LIFE SCI & TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the application of serum miRNA markers in the early screening of OPLL. If serum miRNAs abnormally expressed in OPLL can be screened as biomarkers and corresponding screening kits can be developed, It will be of great significance to the early diagnosis of ossification of the posterior longitudinal ligament in our country

Method used

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  • Serum miRNA markers suitable for diagnosis of ossification of posterior longitudinal ligament and application thereof
  • Serum miRNA markers suitable for diagnosis of ossification of posterior longitudinal ligament and application thereof
  • Serum miRNA markers suitable for diagnosis of ossification of posterior longitudinal ligament and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Research object

[0038] The posterior longitudinal ligament tissues (n=3) of patients with ossification of the posterior longitudinal ligament (n=3) and the normal posterior longitudinal ligament tissues (n=3) during trauma surgery were selected for high-throughput sequencing and bioinformatics analysis of the miRNA group. There was no statistically significant difference between the case group and the control group in age, smoking, drinking and other factors (P>0.05).

[0039] 2. Research Methods

[0040] 1. Select the patient's ligament tissue during the operation, and immediately transfer it to RNAlater (Ambion company or other equivalent products) for short-term storage at low temperature. Freeze at -80°C immediately after transport to the laboratory.

[0041] 2. After all the samples are collected, the preserved tissue samples will be handed over to the company (high-throughput sequencing companies such as Libing Information, Bohao Biology, etc.) for high-thr...

Embodiment 2

[0048] Example 2: Serum OPLL-specific miRNA screening for admitted patients

[0049] 1. Research object

[0050] The case group consisted of 36 cases collected in Shanghai Changzheng Hospital from June 2015 to September 2015, all of whom were diagnosed with ossification of the posterior longitudinal ligament by CT three-dimensional reconstruction of the cervical or thoracic spine, without any treatment before blood collection. The control group consisted of 30 healthy individuals undergoing community disease screening during the same period, and all samples were informed of the purpose of collection and consented to by the patients before collection. There was no statistically significant difference between the case group and the control group in age, smoking, drinking and other factors (P>0.05).

[0051] 2. Research Methods

[0052] 1. Get up in the morning on an empty stomach, use EDTA anticoagulant tube to collect 5ml of cubital venous blood, shake gently after blood coll...

Embodiment 3

[0081] Example 3: Screening patients for OPLL by detecting serum OPLL-specific miRNA

[0082] 1. Research object

[0083] The case group consisted of 68 cases of OPLL collected in the First Spine Department of Shanghai Changzheng Hospital from June 2015 to October 2015, all of whom were diagnosed with ossification of the posterior longitudinal ligament by three-dimensional reconstruction of cervical spine CT, without any treatment before blood collection. The control group consisted of 45 outpatients from the orthopedic department, and none of the 45 patients had obvious symptoms, signs or imaging findings of OPLL. The purpose of collection was informed and the consent of the patients was obtained before all samples were collected. There was no statistically significant difference between the case group and the control group in age, smoking, drinking and other factors (P>0.05).

[0084] 2. Research Methods

[0085] With embodiment 2.

[0086] In order to clarify the specif...

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Abstract

The invention relates to the technical field of medical biological detection, and particularly relates to serum miRNA markers suitable for early screening and diagnosis of ossification of the posterior longitudinal ligament and an application thereof in preparation of a diagnostic reagent or kit for ossification of the posterior longitudinal ligament. The biological markers miRNA-563, miRNA-196b, miRNA-10a and miRNA-129 having relatively high diagnosis value on ossification of the posterior longitudinal ligament are found out for the first time. Through development and application of the serum miRNA markers and the diagnostic kit, early screening of ossification of the posterior longitudinal ligament can be more convenient and easier to implement, the foundation is laid for clinicians to quickly and accurately grasp patient conditions and for improving the clinical treatment effect, and help is provided for finding out novel small-molecular drug targets having potential therapeutic value.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a serum miRNAs marker suitable for early screening and diagnosis of ossification of the posterior longitudinal ligament and its detection application. Background technique [0002] Ossification of the posterior longitudinal ligament (OPLL) is one of the common spinal disorders. Ossifications cause spinal cord compression, causing sensory, motor, and sphincter dysfunction in the limbs and trunk of the patient, eventually leading to quadriplegia and incontinence, which is a serious hazard. Diseases of human health. OPLL is especially common in Asian populations, and the incidence rate in Japan is 1.9-4.3% (StapletonCJ, PhamMH, AttenelloFJ, et al. Ossification of the posterior longitudinal ligament: genetics and pathophysiology [J]. Neurosurg Focus, 2011, 30(3): E6.). Due to the hardness of the ossification material, the risk of decompression surgery is high, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/178C12Q2600/158C12Q1/68
Inventor 刘洋徐辰张浩袁文曹鹏吴卉乔陈元元沈晓龙
Owner SHANGHAI YUHUA LIFE SCI & TECH DEV CO LTD
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