A kind of cross-linked hydrogel based on polyamino acid molecules and its preparation method
A technology for cross-linking hydrogels and polyamino acids, applied in the field of polyamino acids, can solve the problems of obstacles, weak mechanical strength, insufficient toughness, etc., and achieve the effects of wide sources, improved biocompatibility, and simple and easy synthesis methods.
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Embodiment 1
[0047] (1) Add 10.00g of γ-benzyl-L-glutamate and triphosgene to 200mL of tetrahydrofuran. The molar ratio of γ-benzyl-L-glutamate to triphosgene is 1:0.33 , Stir and heat at 55°C for 4 hours. After the completion of the reaction, the crude product was purified by recrystallization to obtain white crystals (N-carboxy-intracyclic anhydride compound) (NCA).
[0048] (2) Dissolve 7.00g of white crystals (N-carboxy-intracyclic anhydride compound) and n-hexylamine in dimethylformamide, and the ratio of the initiator to the N-carboxy-intracyclic acid anhydride compound is 1 : 50, 30℃, under inert gas protection, react for 36 hours. After the completion of the reaction, the solution was distilled under reduced pressure to remove the solvent, and then the concentrated solution was precipitated in ether and dried under vacuum to obtain a white solid-poly(γ-benzyl-L-glutamic acid benzyl ester) (PBLG).
[0049] (3) Dissolve 4.00g white solid-poly(γ-benzyl-L-glutamic acid benzyl ester) in 3-...
Embodiment 2
[0053] (1) Add 10.00g of γ-benzyl-L-glutamate and triphosgene to 200mL of tetrahydrofuran, and the molar ratio of γ-benzyl-L-glutamate to triphosgene is 1:0.40 , Stir and heat at 45°C for 5 hours. After the reaction, the solvent was removed with a rotary evaporator, and the crude product was purified by recrystallization to obtain white crystals (N-carboxyl-cyclic anhydride compound) (NCA).
[0054] (2) Dissolve 7.00g of white crystals (N-carboxy-intracyclic anhydride compound) and n-hexylamine in dimethylformamide, and the ratio of the initiator to the N-carboxy-intracyclic acid anhydride compound is 1 : 100, 50 ℃ under inert gas protection conditions, reaction for 24 hours. After the reaction, the solution was distilled under reduced pressure to remove the solvent, and then the concentrated solution was precipitated in the poor solvent ether, and the white solid was obtained by centrifugation. The final product was white solid-poly(γ-benzyl-L). -Benzyl glutamate) (PBLG).
[00...
Embodiment 3
[0059] (1) Add 10.00g of γ-benzyl-L-glutamate and triphosgene to 200mL of tetrahydrofuran. The molar ratio of γ-benzyl-L-glutamate to triphosgene is 1:0.48 , Stir and heat at 35°C for 6 hours. After the reaction, the solvent was removed with a rotary evaporator, and the crude product was purified by recrystallization to obtain white crystals (N-carboxyl-cyclic anhydride compound) (NCA).
[0060] (2) Dissolve 7.00g of white crystals (N-carboxy-intracyclic anhydride compound) and n-hexylamine in dimethylformamide, and the ratio of the initiator to the N-carboxy-intracyclic acid anhydride compound is 1 : 200, 50℃, under inert gas protection, react for 36 hours. After the reaction, the solution was distilled under reduced pressure to remove the solvent, and then the concentrated solution was precipitated in the poor solvent ether, and the white solid was obtained by centrifugation. The final product was white solid-poly(γ-benzyl-L). -Benzyl glutamate) (PBLG).
[0061] (3) Dissolve 4...
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