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Application of Decanediol as Nitrification Inhibitor

A decanediol preparation technology, which is used in the application field of decanediol as a nitrification inhibitor, can solve the problems of short timeliness and easy loss, and achieve the effects of short timeliness, difficulty in loss, and stable chemical structure

Active Publication Date: 2019-02-19
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrification inhibitory substance of the present invention is a fat-soluble substance with a stable chemical structure derived from plants, which improves the problems of easy loss, short timeliness and possible environmental pollution of traditional nitrification inhibitors, improves nitrification inhibition efficiency, and saves relative investment cost

Method used

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  • Application of Decanediol as Nitrification Inhibitor
  • Application of Decanediol as Nitrification Inhibitor
  • Application of Decanediol as Nitrification Inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Dose-effect relationship of 1,9-decanediol

[0020] 1.1 Experimental design

[0021] (1) Standard product: 1,9-decanediol standard product, customized by WuXi AppTec New Drug Development Co., Ltd. (WuXiAppTec), during the process of dry ice freezing (viscous liquid substance at room temperature), weigh the solid powder and dissolve it in DMSO middle.

[0022] (2) Microbial strain: Nitrosomonas europaea (NBRC 14298=ATCC19718), purchased from Biological Resource Center, NITE, Japan.

[0023] (3) Microbial medium: HEPES medium, containing (NH 4 ) 2 SO 4 2.5g, KH 2 PO 4 0.5g, HEPES11.92g, NaHCO 3 0.5g, MgSO 4 ·7H 2 O 100mg, CaCl 2 2H 2 O 5mg, Fe-EDTA 75mg, pH 7.8-8.0.

[0024] (4) Microbial cultivation: N. europeanum was inoculated in HEPES medium, cultured by shaking at 30° C., 200 rpm, and in the dark (aerobic), and entered a stable period 7-9 days after each transfer.

[0025] (5) Nitrosation activity inhibition experiment

[0026] Collect the cul...

Embodiment 2

[0030] Example 2 Comparison of nitrosation inhibition ability between 1,9-decanediol and other nitrification inhibitors

[0031] 1.1 Experimental design

[0032] (1) Standards: 2-chloro-6-trichloromethylpyridine (Nitrapyrin), dicyandiamide (DCD), 2-amino-4-chloro-6-methylpyrimidine (AM), p-hydroxyphenylpropionic acid Methyl ester, methyl coumarate, linoleic acid, linolenic acid, and methyl linoleate, among which methyl coumarate was purchased from TCI (Tokyo, Japan), and the rest of the reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the above reagents are soluble in DMSO except DCD which is soluble in water.

[0033] (2) DCD is soluble in water, and DMSO is not added to the blank, and other experimental operation steps are the same as in Example 1.

[0034]1.2 Experimental results

[0035] The experimental results are shown in Table 1, ED of 1,9-decanediol 80 The value is similar to synthetic nitrification inhibitor AM, higher than dicyandiamide, low...

Embodiment 3

[0038] Example 3 Comparison of the Nitrosation Inhibition Ability of 1,9-Decanediol and Its Isomers

[0039] 1.1 Experimental design

[0040] (1) Standard products: 1,10-decanediol and 1,2-decanediol were purchased from TCI Company (Tokyo, Japan), and dissolved in DMSO.

[0041] (2) The experimental operation steps are the same as in Example 1.

[0042] 1.2 Experimental results

[0043] See the experimental results image 3 , isomers of 1,9-decanediol at 100μg·mL -1 It has good nitrosation inhibitory ability at all concentrations, and medium and long-chain saturated alcohols such as 1,9-decanediol have good application prospects as nitration inhibitors.

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Abstract

The invention discloses a use of decanediol as a nitrification inhibitor. The decanediol has high efficiency nitrosation microbe inhibition activity at a low dosage, reduces a nitrogen element loss and environment problems caused by farmland soil nitrification. The decanediol is a liposoluble compound, has a stable chemical structure, does not easily lose in soil and water and guarantees application stability and high effectiveness. The green biological nitrification inhibitor is derived from plants, solves the problem that the nitrification inhibitor obtained through conventional nitrification inhibitor chemical synthesis method has short time efficiency, poor effects and pollution easiness, and provides a novel method and thinking for construction of a biologic technology of efficiently inhibiting a farmland ecosystem nitrification process and improving a nitrogen utilization rate.

Description

technical field [0001] The invention belongs to the research field of nitrification inhibitors, and in particular relates to the application of decanediol as a nitrification inhibitor. Background technique [0002] 90% of nitrogen fertilizers worldwide are NH 4 + administered in the form of, NH 4 + Easily converted to NO by nitrifying microorganisms in soil 3 - , the latter cannot be absorbed by the soil and is easy to leaching, which leads to the direct loss of nitrogen, and also seriously pollutes groundwater and surface water, causing environmental problems such as non-point source pollution. At the same time, soil denitrifying microorganisms denitrify NO under anaerobic or partially anaerobic conditions. 3 - into gaseous N 2 and N 2 O, causing further loss of soil nitrogen, while N 2 O is a greenhouse gas with a warming potential of CO 2 One of the important sources is the nitrification and denitrification process of nitrogen in the farmland ecosystem. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/08C05G3/90
CPCY02P60/21
Inventor 施卫明孙力陆玉芳
Owner INST OF SOIL SCI CHINESE ACAD OF SCI