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A kind of aspergillus fumigatus and application thereof

A technology of Aspergillus fumigatus and preservation number, applied in the field of microorganisms, can solve the problems of rare reports of pyrene-degrading molds, teratogenicity, limited microbial resources, etc.

Inactive Publication Date: 2019-05-28
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The pyrene benzene rings of PAHs are symmetrically arranged and stable in structure. They are representative compounds of high molecular weight PAHs and have carcinogenic and teratogenic structural domains. Therefore, the microbial resources that can degrade pyrene are still very limited, especially the relevant research on pyrene-degrading molds is currently more rarely reported

Method used

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  • A kind of aspergillus fumigatus and application thereof
  • A kind of aspergillus fumigatus and application thereof
  • A kind of aspergillus fumigatus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Isolation and identification of pyrene degrading bacteria

[0025] 1. Medium formulation

[0026] PDA medium: potato extract 200g / L, glucose 20g / L, agar 15-20g / L.

[0027] Liquid medium: slightly improved on the basis of the formula of Tien and Kirk (1988): the concentration of which is 0.02mol·L -1 acetic acid buffer (pH=4.4) instead of dimethyl succinate buffer. Among them, glucose 10g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 0.25g / L, CaCl 2 0.1g / L, ammonium tartrate 0.5g / L (C 4 H 12 N 2 O 6 ), trace elements 10g / L, the composition of trace elements ( / L):

[0028] MnSO 4 0.5g, NaCl 1.0g, FeSO 4 ·7H 2 O 0.1g, COCl 2 0.1g, ZnSO 4 ·7H 2 O 0.1g, CuSO 4 0.1g, AlK(SO 4 ) 2 ·12H 2 O 0.01g, Na 2 MoO 4 ·2H 2 O 0.01g

[0029] The VB1 solution was added by sterile filtration before inoculation, so that the final mass concentration of VB1 was 5 mg / L.

[0030] Zha's medium formula: 30.0g / L sucrose, 3g / LNaNO 3 , 1g / LK 2 HPO 4 , 0.5g / LMgSO 4 ·7H ...

Embodiment 2

[0049] Example 2 Degradation ability of Aspergillus fumigatus H1 to pyrene

[0050] 1. Preparation of primary seeds

[0051] First inoculate on the PDA medium from the slant surface, grow in the incubator at 30°C for more than 10 days, and when the surface is covered with white spores, gently scrape the spores on the surface with a sterile spoon, and place it in a culture solution containing 100 mL of sterile inorganic salts. Medium, 160r / min, 30 ℃ culture for 3-5 days, when the spore ball grows to about 2mm, it enters the rapid growth period, and it is used as a first-class seed.

[0052] 2. Experimental procedure

[0053] Inoculate 5 mL of spore liquid with a sterile pipette and inoculate it into 25 mL of liquid medium containing pyrene, and seal the bottle with a sterile sealing culture film (the sterile sealing culture film is a double-layer film produced by Huankai Company, with a diameter of 0.6 cm on it. There are two round holes in the middle of the two membranes, an...

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Abstract

The invention discloses an aspergillus fumigatus and application thereof. The Aspergillus fumigatus of the present invention is Asperqillus fumigatus H1, and its preservation number is CGMCC No.11576. The Aspergillus fumigatus of the present invention can be used to degrade pyrene, and further be used to degrade polycyclic aromatic hydrocarbons. The aspergillus fumigatus of the invention can effectively and rapidly degrade pyrene and other polycyclic aromatic hydrocarbons, and provide powerful assistance for the biological treatment engineering of pyrene pollution and other polycyclic aromatic hydrocarbon pollution.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular, to an Aspergillus fumigatus and its application. Background technique [0002] PAHs are composed of two or more benzene rings in a linear arrangement, bending or clustering. Generally can be divided into two categories, namely isolated polycyclic aromatic hydrocarbons and fused polyaromatic hydrocarbons. Condensed polycyclic aromatic hydrocarbons pose a great threat to human beings, and the polycyclic aromatic hydrocarbons with carcinogenic effects are mostly four to six ring fused ring compounds. Most PAHs are insoluble in water, the melting point is as high as 101℃~438℃, the boiling point is 150℃~525℃, and the molecular weight is between 178~300. Bicyclic and tricyclic PAHs are easy to degrade, and PAHs with more than four rings are extremely difficult to degrade. The results show that: in indoor environment, the half-life of bicyclic PAHs is 2 days; the half-life of tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/68A62D101/20
CPCA62D3/02A62D2101/20C12N1/145C12R2001/68
Inventor 黄赛花侯梅芳周建赵海青董浩
Owner SHANGHAI INST OF TECH
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