Device and method for on-line detection of small organic molecules in plants

Abstract

The invention provides an on-line detection device of small organic molecules of plants and an on-line detection method of small organic molecules by the utilization of the above device. The above device comprises a microbiosensor unit, an excitation signal generating unit, a data acquisition and processing unit and a main control unit. The main control unit generates an original excitation signal to the excitation signal generating unit such that the excitation signal generating unit generates an excitation signal; the microbiosensor unit generates an electric signal corresponding to concentration of small organic molecules to be detected under the action of the excitation signal; the electric signal is pretreated by the data acquisition and processing unit; and afterwards, the main control unit analyzes the pretreated electrical signal so as to obtain the concentration of the small organic molecules to be detected. When the device is in use, the microbiosensor unit is inserted into a plant organ to be detected to carry out detection. Thus, on-line, in-vivo and dynamic detection of plants' small organic molecules and continuous analysis are realized. The invention provides a new solution for phytophysiology, plant electrophysiology, botany, phytocytobiology and the like.

Description

technical field [0001] The invention belongs to the technical field of organic matter monitoring, and more specifically relates to an online detection device and method for small organic molecules in plants. Background technique [0002] Plant organic small molecules play an important role in plant growth and development. Existing plant organic small molecule detection equipment includes amino acid analyzers, microplate readers, gas chromatography-mass spectrometers, high-performance liquid chromatography analyzers, etc. These detection equipments are all detected by analytical chemistry methods, and the detection process requires in vitro analysis, and samples are taken at different time points, and then through pre-treatment processes such as grinding, homogenization, and filtration, and finally the organic small molecule content is analyzed on the machine. [0003] The existing detection technology detects the static concentration of small organic molecules in plants at o...

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Problems solved by technology

[0003] The existing detection technology detects the static concentration of small organic molecules in plants at one or several time points, which requires in vitro detection, which can only obtain static information of small organic molecules in plants, and cannot reflect the dynamic response mechanism of plants to environmental changes. , and the preparation and pretreatment process of the detection sample is complicated and the efficiency is low

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