Mixed stem cell-based medicinal product and preparation method thereof

A technology of mixing stem cells and stromal cells, applied in the field of stem cell preparations and their preparation, can solve the problems of cell number and expansion, decreased differentiation ability, and unsuitable for batch preparation, etc., to promote proliferation and maintain stem cell characteristics, and promote epidermal cell growth. , the effect of shortening the wound healing time

Inactive Publication Date: 2016-04-13
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Bone marrow-derived mesenchymal stem cells are not suitable for batch production due to the hidden danger of virus contamination,

Method used

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Embodiment Construction

[0022] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only the present invention. Some embodiments of the invention are not all of the embodiments.

[0023] The invention provides a mixed stem cell preparation, which is to mix epidermal stem cells and hair follicle mesenchymal stem cells, and add low-molecular-weight heparin sodium, IL-6, IL-3, SCF, 4Flt-3L, chitosan and hemoglobin saline.

[0024] In the embodiment of the present invention, the concentration of epidermal stem cells is 5~7×10 5 each / ml, the concentration of the hair follicle mesenchymal stem cells is 1~3×10 5 pieces / ml. More preferably, the concentration of the epidermal stem cells is 6×10 5 individual / ml, the concentration o...

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Abstract

The invention provides a mixed stem cell-based medicinal product and a preparation method thereof. The mixed stem cell-based medicinal product comprises 5-7*10<5>/ml epidermal stem cells and 1-3*10<5>/ml hair follicle mesenchymal stem cells, and 15-20ng/ml low-molecular-weight heparin sodium, 10-20ng/ml IL-6, 15-25ng/ml IL-3, 45-55ng/ml SCF, 45-55ng/ng Flt-3L, 5-20ng/ml chitosan and 5% (WT) hemalbumin normal saline are added into the mixed stem cell-based medicinal product. The mixed stem cell-based medicinal product and the preparation method have the advantages that the mixed stem cell-based medicinal product is prepared by mixing the epidermal stem cells with the hair follicle mesenchymal stem cells, is capable of promoting epidermal cell growth, shortening wound healing time, promoting proliferation and maintaining stem cell characteristics and is superior to single-cell medicinal products in effect on promoting chronically unhealed ulcer healing and the like.

Description

technical field [0001] The invention relates to a stem cell preparation and a preparation method thereof, in particular to a stem cell preparation for treating burnt skin and a preparation method thereof. Background technique [0002] It has been reported that wound coverage after severe trauma, extensive burns, and plastic surgery has become a very important issue. In addition to autologous skin grafts, people have used allogeneic skin grafts and other related repair materials to cover wounds, but Both autologous and allogeneic skin transplantation are limited by donor source and immune rejection. It is urgent to find an ideal natural medicine without rejection, promoting skin damage repair, and promoting skin regeneration. [0003] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. A large number of experimental studies have shown that mesenchymal stem cells have the ...

Claims

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Application Information

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IPC IPC(8): A61K38/42A61K38/20A61K38/19A61K35/28A61K35/36C12N5/0775C12N5/074A61P17/02A61K31/722A61K31/727
CPCA61K35/28A61K31/722A61K31/727A61K35/36A61K38/19A61K38/193A61K38/202A61K38/204A61K38/42C12N5/0625C12N5/0666
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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