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Longan embryonic development related gene dliku1 and its application

A technology of embryo development and longan, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2018-09-11
POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a longan embryo development-related gene DlIKU1 and its application for the deficiency that the existing longan embryo development gene has not been cloned

Method used

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  • Longan embryonic development related gene dliku1 and its application
  • Longan embryonic development related gene dliku1 and its application
  • Longan embryonic development related gene dliku1 and its application

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Embodiment Construction

[0036] 1. Cloning of DlIKU1, a gene related to embryonic development of longan

[0037] Design primers based on the Arabidopsis IKU1 conservative region sequence to amplify the cDNA of normal developing longan (FJ7811). Through three rounds of PCR amplification, a 1100bp amplified fragment was obtained. The ORF Finder software was used to predict the longan DlIKU1 open reading frame (ORF) It is composed of 990 bases; it encodes a protein with 329 amino acids and an isoelectric point (pI) of 11.45.

[0038] The protein structure prediction shows that DlIKU1, like Arabidopsis IKU1, contains a conserved VQ motif domain ( figure 2 ).

[0039] The amino acid sequence of homologous genes in DlIKU1 and 5 reference species (Citrus sinensis, Citrusclementina, Populus trichocarpa, Ricinus communis, Vitisvinifera) A multiple sequence alignment was performed, and the results showed that: DlIKU1 and the isoprotein sequence both contain the same conserved domains and have certain homology ( ima...

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Abstract

The invention discloses a longan embryonic development related gene DlIKU1 and application thereof, and belongs to the field of plant genetic engineering. A longan DlIKU1 open reading frame of the longan embryonic development related gene DlIKU1 is totally composed of 990 basic groups; protein with 329 amino acids and the isoelectric point being 11.45 is encoded. A cloning method comprises the steps that a primer is designed according to an arabidopsis IKU1 conserved sequence, the cDNA of normally-developed longan is amplified, 1100 bp amplified fragments are obtained through three-round PCR amplification, and it is predicted that the longan DlIKU1 open reading frame is totally composed of the 990 basic groups through ORF Finder software; the protein with the 329 amino acids and the isoelectric point being 11.45 is encoded. The longan DlIKU1 gene can be used for controlling longan embryonic development by inhibiting the expression of the longan DlIKU1 gene, and stable aborted-seeded longan is obtained. The longan embryonic development related gene DlIKU1 has the quite high application value in production for cultivating high-quality longan varieties with high-quality small-seed longan.

Description

Technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to a longan embryo development related gene DlIKU1 and its application. Background technique [0002] Longan (Dimocarpus Longan Lour.), also known as longan, is a precious subtropical fruit tree in my country. It is widely distributed in Fujian, Guangdong, Guangxi and other provinces. Its fruit has high economic value and is rich in vitamins, fructose and minerals, which are beneficial to the human body. Ingredients, and have certain medicinal and health effects. With the gradual development of longan-related industries, the characteristics of sweetness and small nucleus have become the criteria for screening longan varieties (Gu Xiaoling, Li Yuping, Liang Weihong, etc. Overview of the development of China's longan industry. Chinese Agricultural Science Bulletin, 2008, 09:470-474 ). [0003] For fruit trees, the small nucleus or seedless produced by embryo abortion can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/10A01H5/00
CPCC07K14/415C12N15/8267
Inventor 姜帆田真真郑少泉陈亮
Owner POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI