A method for rapidly propagating golden iron lock seedlings
A kind of seedling and rapid technology, applied in the field of rapid propagation of Jintie lock seedlings, can solve the problems of tissue culture seedling pollution, slow development speed, high production cost, etc., and achieve the effect of meeting market demand, increasing development speed, and reducing seedling cost
Active Publication Date: 2017-10-10
YUNNAN LYUCHEN BIOTECH DEV CO LTD
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Problems solved by technology
However, the conventional seed raising method has a long seedling cycle and a low reproduction coefficient, resulting in high production costs and slow development.
In order to solve the existing problems of conventional seed seedling cultivation, some began to study using tender stem tissue culture, as Chinese patent application CN104255489A disclosed "Jintie lock belt axillary bud tender stem asexual rapid propagation technology method", but the tender stem tissue has endophytes, tissue Endophytic bacteria will cause contamination of tissue culture seedlings during cultivation, resulting in low seedling rate and poor quality of seedlings
Method used
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Embodiment Construction
[0013] 1. Seed treatment: choose mature, plump and dry Jintiesuo seeds, soak them in washing powder solution for 20-30 minutes, rinse them with water for 3-5 times, then soak them in 70%-75% alcohol for 20-30s, Rinse with sterile water 2 to 3 times, and then use 0.1% to 0.2% HgCl 2 After soaking for 6-8 minutes, rinse with sterile water for 5-7 times, and finally dry the water with sterile filter paper;
[0014] 2. Protocorm induction: Inoculate the Jintiesuo seeds treated according to step 1 in the protocorm induction medium, and cultivate them in the dark for 8 to 10 days at a daytime temperature of 18-23°C and a night temperature of 15-20°C, and the seeds will start to germinate Swell, adjust the cultivation environment at this time, and cultivate the germinated and expanded seeds for 6 to 8 days at a temperature of 24-26°C in the daytime and 19-21°C in the night, with a light of 1200-1500Lx and a daytime light duration of 10-12h. Form a protocorm with a diameter of 2-3mm....
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The invention provides a method for rapidly propagating Psammosilene tunicoides seedlings. The method comprises seed treatment, protocorm induction, protocorm multiplication culture, cluster bud induction, cluster bud multiplication culture and strong seedling culture. The method is characterized in that a protocorm induction culture medium is inoculated with disinfected Psammosilene tunicoides seeds for culture, protocorms with the diameter of 2-3 mm are formed and transferred to a protocorm multiplication culture medium for culture after being cut, a large number of protocorms with the diameter of 2-3 mm are obtained, the protocorms are transferred to a cluster bud induction culture medium for culture, a large number of cluster buds with the length of 1-2 mm are obtained and transferred to a multiplication culture medium for culture, a large number of cluster buds with the length of 10-20 mm are obtained and divided into single buds to be transferred to a strong seedling culture medium for culture, and a large number of detoxified Psammosilene tunicoides seedlings with the length of 80-100 mm are obtained. With the adoption of the method for propagating the Psammosilene tunicoides seedlings, the seedling culture period is short, the propagation coefficient is high, and the seedling quality is good.
Description
technical field [0001] The invention relates to a method for rapidly propagating jintiesuo seedlings, in particular to a method for rapidly propagating jintiesuo seedlings with jintiesuo seeds. Background technique [0002] Psammosi lene tunicoi des W.C.Wu etc.y.wu, also known as Psammosi lene tunicoi des W.C.Wu etc.y.wu, is a plant of the genus Psammosi lene tunicoi in the Caryophyllaceae family. , It is often used clinically to treat rheumatoid arthritis and other diseases. The main chemical components of Jin Tiesuo are triterpenes, triterpene glucosides, cyclic peptides and lactams. Pharmacological tests have shown that the analgesic and anti-inflammation effects of total glucoside are obvious. In the past, it was mainly bred in the wild, but in recent years, due to the increasing demand for it, wild plant resources have declined sharply, and it has become a rare and endangered species. It has been listed as a national second-class protected plant in the "Chinese Plant R...
Claims
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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 寸建清
Owner YUNNAN LYUCHEN BIOTECH DEV CO LTD