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Fully automatic non-centrifugal closed cell washing system

A cell and washing device technology, which is applied in the field of medical devices, can solve problems such as unsatisfactory effects, and achieve the effects of high biological safety performance, high washing efficiency, and small cell damage

Active Publication Date: 2018-05-25
SHANGHAI BAIZE MEDICAL APP & INSTR CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has the advantages of high washing efficiency and relatively simple operation. It is suitable for washing large volumes of blood cells, but the effect is not ideal when dealing with small volumes (0.5ml-1ml) of frozen cells.

Method used

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  • Fully automatic non-centrifugal closed cell washing system
  • Fully automatic non-centrifugal closed cell washing system
  • Fully automatic non-centrifugal closed cell washing system

Examples

Experimental program
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Embodiment

[0105] In this embodiment, 1ml-1.5ml of hybridoma cryopreserved cell suspension is processed. 10ml of PBS washing buffer was added to the mixing module 5 in advance. The tubing uses a disposable biocompatible tubing with an inner diameter of 2 mm and an outer diameter of 3 mm. The filter chamber 14 uses a microporous filter membrane with a pore size of 0.7um, the size of the membrane is 12.5mm×40mm, and the volume of the filter chamber 14 is 0.5ml. Add 1ml of frozen cell suspension to the mixing module 5 to start the whole cycle washing process. The pinch peristaltic pump speed of the drive module 6 is controlled at 30r / min. When washing is finally completed, about 0.5 ml of the washed cell mixture can be recovered. Finally, after cell counting, the experimental results of cell washing are shown in Table 1, and the final cell yield can reach more than 80%, indicating that it has a good effect in processing small volume samples, compared with the traditional centrifugation m...

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Abstract

The invention relates to a full-automatic non-centrifugal closed cell washing system. Particularly, the invention relates to a washing and filtering module, which comprises a filter cavity, a liquid waste cavity, a sample liquid inlet, a sample liquid outlet, a liquid waste outlet and a filter membrane, wherein the sample liquid inlet and the sample liquid outlet are communicated with the filter cavity; the liquid waste outlet is communicated with the liquid waste cavity; the filter membrane is used for separating the filter cavity and the liquid waste cavity; and the filter cavity and the liquid waste cavity are only communicated with the outside through the sample liquid inlet, the sample liquid outlet and the liquid waste outlet. The invention further relates to a device comprising the washing and filtering module for washing cells. The device provided by the invention is capable of washing the cells with large volume dose, and especially has a good effect on washing of small-volume frozen cells; and the recycled cell suspension can be directly applied clinically.

Description

technical field [0001] The invention belongs to the field of medical equipment, and in particular relates to a fully automatic non-centrifugal closed cell washing system. Background technique [0002] At present, in the fields of medicine and physiology, cell cryopreservation technology is mainly used for long-term storage of specific cells (such as lymphocytes, blood cells, etc.). This technology mostly uses glycerol or dimethyl sulfoxide (DMSO) as a protective agent to cryopreserve together with the cells to improve the water permeability of the cell membrane and reduce the generation of intracellular ice crystals during the cryopreservation process, thereby reducing cell damage. When thawing cells, it is generally necessary to wash the frozen cells with a buffer to remove harmful components such as glycerol or dimethyl sulfoxide. In addition, cell washing technology is also widely used in other occasions. For example, hospitals and blood stations need to wash human red ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/12C12M1/00
CPCC12M23/28C12M41/48C12M47/04
Inventor 钱其军周进师传胤孙紫盎庞震国颜开冬吕功路
Owner SHANGHAI BAIZE MEDICAL APP & INSTR CO LTD
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