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SSR labeling primer of flammulina velutipes F101 culture and fingerprint application thereof

A technology of labeling primers and fingerprints, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of mushroom farmers' loss, influence on cultivation enthusiasm, and influence on the development of Flammulina velutipes industry, and achieve accurate High performance, good application prospects, and short detection time

Inactive Publication Date: 2016-04-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

As far as the country is concerned, the phenomenon of "same species with different names, different species with the same name" in the cultivation of Flammulina velutipes not only brought great losses to mushroom farmers, but also affected their enthusiasm for cultivation, and also greatly affected the development of China's Flammulina velutipes industry ; And, along with the appearance of large-scale industrialized cultivation mode, the requirement to the quality of Flammulina velutipes cultivation strain is more and more higher, need to develop more simple, fast, accurate bacterial strain identification technology, to guarantee that each batch uses kind accurate

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  • SSR labeling primer of flammulina velutipes F101 culture and fingerprint application thereof
  • SSR labeling primer of flammulina velutipes F101 culture and fingerprint application thereof
  • SSR labeling primer of flammulina velutipes F101 culture and fingerprint application thereof

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[0048] In this example, combined with the Figure 1-7 , describe the present invention in detail.

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Abstract

The invention discloses an SSR labeling primer of a flammulina velutipes F101 culture and fingerprint application thereof. The labeling primer is composed of at least one of six pairs of SSR primers in Fvm1, Fvm2, Fvm3, Fvm4, Fvm5 and Fvm6. The method comprises the steps of 1, hypha solid culture, 2, genome DNA extraction, 3, SSR molecular marker detection, 4, electrophoresis detection and 5, strain identification. Compared with conventional morphological detection, antagonistic tests and fruiting tests, SSR labeling has the advantages of being short in detection time, high in accuracy and good in repeatability.

Description

technical field [0001] The invention relates to the field of strain detection and identification, in particular to an SSR-labeled primer of Flammulina velutipes F101 strain and application thereof. Background technique [0002] Flammulina velutipes (Fr.) Sing. belongs to the Phylum Fungi, Basidiomycotina, Laminomycetes, Agarobasidiomycetes, Agaricaceae, Tricholomaceae, and Flammulina genus. It is named for its shape like day lilies. Flammulina velutipes is also known as structure bacteria, enoki mushrooms, velvet mushrooms, hairy stalk money bacteria and winter mushrooms. The natural distribution of Flammulina velutipes is very extensive. As a kind of edible fungus, Flammulina velutipes is one of the very popular natural health foods in the market today. Flammulina velutipes has high protein content, rich amino acid types, and has anti-cancer functions. Regular consumption can prevent high blood pressure, treat liver diseases and gastric ulcers. Flammulina velutipes is es...

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 沈颖越金群力蔡为明宋婷婷范丽军冯伟林李良英田芳芳
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES