Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sperm cryoprotectant and preparation method thereof

A cryoprotectant and cryopreservation technology, which is applied to the preservation, application, and animal husbandry of human or animal bodies, can solve problems such as infection and complex components of cryoprotectants, and achieve the effect of simple preparation methods

Inactive Publication Date: 2016-05-04
REPRODUCTIVE & GENETIC HOSPITAL OF CITIC XIANGYA CO LTD
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the sperm cryoprotectants used in various sperm banks and reproductive centers are mostly formulated with egg yolk, glycerin, and sucrose. Since egg yolk must be added, the composition of the cryoprotectant is quite complicated, and there is a risk of animal-derived pathogenic bacteria infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sperm cryoprotectant and preparation method thereof
  • Sperm cryoprotectant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of cryoprotectant for sperm, its formula composition and content are as follows:

[0029] Sucrose 16g, sodium bicarbonate 0.5g, glycerin 36mL, 20% (g / mL) human albumin 25mL, five-distilled water 60mL.

[0030] The preparation method of above-mentioned sperm cryoprotectant comprises the following steps:

[0031] 1. Weigh 16g of sucrose and 0.5g of sodium bicarbonate on an electronic balance;

[0032] 2. Measure 36mL of glycerin, 25mL of 20% (g / mL) human serum albumin, and 60mL of five-distilled water (made by conventional methods, obtained by distilling water five times) in a graduated cylinder for use;

[0033] 3. First add 60mL five-distilled water into a 250mL beaker, and then put stirring magnetic beads;

[0034] 4. Place the beaker on the magnetic stirrer, first adjust the speed to the lowest, turn on the power, and then adjust the speed to an appropriate speed, so as to maintain a large speed and the liquid in the beaker will not splash out;

[0035] 5. A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a sperm cryoprotectant and a preparation method thereof. Every 125 ml of the sperm cryoprotectant is prepared from saccharose, sodium bicarbonate, glycerol, human serum albumin and water obtained by distilling for five times. The novel sperm cryoprotectant does not contain yolk and only contains saccharose, sodium bicarbonate, glycerol, 20% (g / mL) human serum albumin and other commonly-used reagents with definite chemical components, the risk of animal source pathogenic bacterium infection does not exist, the acquired freezing effect is similar to that of an existing formulation, and the average value of the sperm freezing-thawing rate is about 75% to 80%.

Description

technical field [0001] The invention relates to the field of sperm cryopreservation in assisted reproductive technology, in particular to a sperm cryoprotectant and a preparation method thereof. Background technique [0002] The cryopreservation of human sperm plays an important role in human reproductive health, and the cryoprotectant added during the freezing process is one of the key factors affecting the effect of cryopreservation. [0003] At present, the sperm cryoprotectants used in various sperm banks and reproductive centers are mostly formulated with egg yolk, glycerin, and sucrose. Since egg yolk must be added, the composition of the cryoprotectant is quite complicated, and there is a risk of animal-derived pathogenic bacteria infection. Contents of the invention [0004] Based on this, it is necessary to provide a sperm cryoprotectant and a preparation method thereof in order to solve the above problems. The sperm cryoprotectant of the present invention does no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 朱文兵卢光琇范立青彭向杰
Owner REPRODUCTIVE & GENETIC HOSPITAL OF CITIC XIANGYA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com