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Application of anemoside B4 serving as EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs

A technique of Pulsatilla saponin and hand, foot and mouth disease, which is applied in the field of medicine and achieves the effects of safety, reliability and no toxic and side effects.

Active Publication Date: 2016-05-04
刘琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have found that Pulsatilla saponins have the effects of enhancing immune function, anti-inflammation, anti-tumor, and anti-pathogenic microorganisms, so they have received extensive research and attention, but there are no reports of Pulsatilla saponin monomer compounds with viral activity

Method used

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  • Application of anemoside B4 serving as EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs
  • Application of anemoside B4 serving as EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs
  • Application of anemoside B4 serving as EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Determination of anti-EV71 activity of Pulsatilla saponin B4 in vitro.

[0017] (1) Virus infection

[0018] Infection mode: RD cells (human rhabdomyoma cells), EV71 can infect and replicate in RD cells, causing apoptosis and necrosis of RD cells, EV71 mainly infects human muscle and nerve tissues, and infecting RD cells with EV71 in vitro can simulate hand, foot and mouth disease model.

[0019] Infection method: EV71 infects RD cells to cause RD cell apoptosis and necrosis. RD cells were planted in 6-well plates. After the cells adhered to the wall, the concentration reached 80%. EV71 infected cells, and normal cultured RD cells were used as blank control. Samples were taken at different times after infection, the cytopathic effect (CPE) of the virus was observed with an inverted fluorescence microscope, and the apoptosis and necrosis of the cells were measured by flow cytometry. The results of flow cytometry are shown in the attached instructions figur...

Embodiment 2

[0027] Example 2: Determination of anti-EV71 activity of Pulsatilla saponin B4 in vivo

[0028] (1) Experimental animals: 2-day-old ICR mice with an average body weight of 1.6-1.8 grams.

[0029] (2) Infection method: Take 2-day-old suckling mice and inject EV71 virus intraperitoneally after 5 hours of fasting.

[0030] (3) Experimental grouping: divided into 9 groups: negative group (uninfected virus group), model group (infected with virus, intraperitoneal injection of equal volume of normal saline every day), positive control group (after infected with virus, daily ribavirin Intraperitoneal injection treatment, 100mg / kg / day), experimental group I (after 24 hours of virus infection, intraperitoneal injection of Pulsatilla saponin B41 times a day, the doses were 50, 100, 200mg / kg / day), experimental group II (after 72 hours of virus infection, Daily intraperitoneal injection of Pulsatilla saponin B41 times, the doses were 50,100,200mg / kg / day). There were 12 experimental anim...

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Abstract

The invention discloses application of anemoside B4 serving as an EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs. The application of the anemoside B4 in preparing the hand-foot-and-mouth disease resisting drugs is generated through activity for inhibiting cell apoptosis and necrocytosis caused by EV71 infection, the application of the anemoside B4 serving as the EV71 virus inhibitor in curing the hand-foot-and-mouth disease provided by the invention is disclosed for the first time, and the anemoside B4 is a pure natural preparation, is safe, reliable and free of toxic and side effects, and has potential value in research and development of drugs for curing the hand-foot-and-mouth disease.

Description

technical field [0001] The invention relates to the application of pulsatilla saponin B4 as an EV71 virus inhibitor in the preparation of anti-hand, foot and mouth disease medicine, and belongs to the field of medicine. Background technique [0002] Hand-foot-mouth disease (hand-foot-mouth disease, HFMD) is an acute infectious disease caused by enterovirus that seriously harms infants under 5 years old. More than 10,000 cases. As of the end of 2015, nearly 14 million cases of hand, foot and mouth disease have been reported nationwide, and more than 3,300 infant deaths have been reported. The major impact on stability and economic development has attracted widespread attention. The main manifestations of hand, foot and mouth disease are fever, ulcerative herpes on the oral mucosa, and blister-like rash on the extremities. Some patients have fatal complications such as myocarditis and pulmonary edema due to the virus attacking the heart and brain. Among them, Coxsackie virus ...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P31/14
CPCA61K31/704
Inventor 刘琦
Owner 刘琦
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