Method for preparing anti-fatigue Chinese herb extract
An extract and anti-fatigue technology, applied in the field of preparing anti-fatigue traditional Chinese medicine extracts
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Embodiment 1
[0016] The preparation of embodiment 1 Huanliangcao extract
[0017] Grind 1kg of Huanliangcao dry product into powder, soak it in 4L of 75% by volume methanol aqueous solution and shake it for 1h, then place it at room temperature for 72h, then filter it, and concentrate the filtrate under reduced pressure at 40°C into a thick paste, freeze-dried, crushed into fine powder, and passed through a 100-mesh sieve to obtain the dry powder of Huanliangcao extract.
Embodiment 2
[0020] Example 2 Experimental study on the anti-fatigue effect of Huanliangcao extract on mice
[0021] There were 108 healthy Kunming mice, weighing 18g-22g, male and female. After pre-feeding for 7 days, they were randomly divided into the following three groups, blank control group, test group A, and test group B, with 36 rats in each group. And carry out the following gavage treatment: taking the body weight as a benchmark, the blank control group was gavaged with 0.9% normal saline, and the dosage was 0.15ml / 10g; The Huanliangcao extract prepared in the comparison example was administered to the group at a dose of 1.72 g / kg / d, and each was administered once a day at 9:00 am and 5:00 pm for a total of 30 days. During the experiment, the animals had free access to food and water, and were deprived of food the night before the last gavage.
[0022] (1) Exercise endurance test
[0023] 30 minutes after the last gavage, 12 mice were randomly selected from each group to swim...
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