Method for manufacturing complete section of corn mature seed farinaceous albumen with assistance of nail polish
A production method and technology for silty endosperm, which are applied in the preparation of test samples and other directions, can solve the problems of unwell starch-rich mature seeds, tedious and time-consuming resin embedding process, inability to complete silty endosperm, etc., and achieve obvious contrast. , easy to promote, low toxicity effect
Active Publication Date: 2016-05-04
YANGZHOU UNIV
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Problems solved by technology
At present, the research on the morphology and structure of corn endosperm mainly focuses on developing seeds, but there are few studies on mature seeds, especially the research on floury endosperm. The main reasons are as follows: paraffin sectioning technique, but this method is not suitable for mature starch-rich seeds
②Using resin to embed mature corn seeds, although slices can be made, but the corn seeds are too large to obtain complete endosperm slices, and the resin embedding process is cumbersome and time-consuming, and the embedding agent is expensive
③ Some people in foreign countries use the dry cutting method to slice corn seeds, but it is only suitable for the horny endosperm, while the powdery endosperm cannot be completed, and the dry cutting method cannot obtain complete seed slices
Method used
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[0030] Below in conjunction with example accompanying drawing, the present invention will be further described:
[0031] 1. Preparation of sliced samples:
[0032] Seed pasting and fixing: First, use a file to grind the mature corn grains along the cross-cutting direction to the target section, and then use a sharp blade or wire saw to cross-cut the middle part of the corn seed to obtain a sample piece; use a file on the top of the resin base with a suitable size and uniform shape Grind it flat and wipe it clean with alcohol. After the alcohol dries, add an appropriate amount of super glue, place the sample block horizontally in the glue on the resin base, press lightly for 10 seconds, and leave it overnight to ensure a firm paste. The cross-sectional area of the resin base should be larger than that of the sample to prevent the sample from shaking during slicing. At the same time, the resin base should be smaller than the size of the sample clamp so that the base can be c...
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Abstract
The invention discloses a method for manufacturing a complete section of corn mature seed farinaceous albumen with the assistance of nail polish. The method comprises the steps of seed sticking and fixing, trimming, nail polish embedding, secondary trimming, section semi-thinning, section dyeing, observation and the like. The method is characterized in that super glue is adopted for sticking and fixing a mature seed to a resin base; 2, the colorless and transparent nail polish is used as an embedding agent for rapidly embedding endosperm tissue; 3, a dry cutting technology easy, fast and convenient to operate and applicable to the corn mature seed farinaceous albumen is built, and the complete section of the seed is obtained; 4, the section has multiple purposes, wherein the section can be subjected to iodine solution dyeing or polarized light observation to know the shape and size of starch grains and space distribution of the starch grains in the endosperm tissue. The problem that the complete section of the corn mature seed farinaceous albumen cannot be prepared in the mode of a paraffin section and a resin section is solved, the defect that the farinaceous albumen section cannot be prepared through a dry cutting method is overcome, and therefore technical support is provided for research on the morphological structure of the corn mature seed farinaceous albumen.
Description
technical field [0001] The invention relates to the technical field of microscopic tissue sectioning of plants, in particular to a method for making complete sectioning of corn mature seed floury endosperm. Background technique [0002] Corn is one of the main source crops for human food, animal feed and industrial raw materials. Corn seeds contain a large endosperm, which is an important nutrient storage tissue for corn, and its development and filling determine the weight and quality of the seeds. Corn endosperm is divided into horny and farina, the former endosperm cells are full, while the latter endosperm cells are not full. Starch is the most important storage material of endosperm, and its shape, size and distribution in endosperm tissue have always been a matter of great concern to maize breeders. [0003] To observe the morphological structure of corn endosperm, the seeds must be sliced first. At present, the research on the morphology and structure of corn end...
Claims
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Login to View More IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 韦存虚赵凌霄潘婷王娟
Owner YANGZHOU UNIV