On-line wine making fermentation liquid yeast activity detection device and method based on Raman spectrum

Abstract

The invention discloses an on-line wine making fermentation liquid yeast activity detection device based on a Raman spectrum. The device comprises a pump, a sample pool, a three-way valve, a communication pool, a laser, an optical fiber, a Raman probe, a reflecting mirror and a computer; the laser is connected to the Raman probe through the optical fiber, the head of the Raman probe is connected with the first surface of the sample pool, the reflecting mirror is arranged on the second surface of the sample pool, and the first surface and the second surface are parallel to each other; a spectrometer is connected to the computer through a communication interface; the sample pool is made of a transparent material. The invention further discloses an on-line wine making fermentation liquid yeast activity detection method based on the Raman spectrum. According to the device and method, on-line wine making fermentation liquid yeast activity detection is achieved in the wine making process, the important significance on guiding a brewery to optimize the fermentation process is achieved, and the fermentation liquid yeast activity can be accurately and quickly detected.

Description

technical field [0001] The invention relates to the field of detection and control of the brewing fermentation process, in particular to a Raman spectrum-based online detection device and method for yeast activity in brewing fermentation broth. Background technique [0002] Fermentation is one of the most important and critical links in the winemaking process. The quality of the yeast in the fermentation broth is directly related to the yield and quality of its fermentation products. The quality of yeast is generally evaluated by activity and vitality. Activity refers to the viability of cells, and yeast activity refers to the physiological state of yeast cells, which can also be understood as the adaptability and metabolic ability of living cells to the environment. Yeast activity is an important factor affecting fermentation performance and taste. Only stable and high-activity yeast can brew high-quality wine. Therefore, how to realize the on-line detection of yeast vigor...

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Problems solved by technology

However, this method has not been applied to the quantitative analysis of yeast viability, nor has it been used in the online detection of yeast viability in fermentation broth during the brewing process.

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