Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus

A technology of Hericium erinaceus and primer pairs, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex PCR amplification patterns, unsuitable for species identification, low specificity, etc., and achieve repeatability Good, short detection time, high specificity

Active Publication Date: 2016-05-11
HANGZHOU ACAD OF AGRI SCI
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the common primers used in these molecular marker techniques are not only complicated, but also poor in repeatability and specificity. Therefore, they are not suitable for variety identification. DNA fingerprinting can really be used for the accurate and rapid identification of the Hericium erinaceus species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus
  • Primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus
  • Primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An exploratory study was carried out on the molecular identification of 15 main species of Hericium erinaceus using SRAP molecular marker technology. Extract the hyphae genome DNA of each Hericium erinaceus species as a template, carry out PCR amplification with a large number of SRAP random primer pairs, and detect the amplified products by agarose gel electrophoresis, and finally screen to obtain differential bands (only Hericium erinaceus L. A pair of primers for No. 2 and Hericium erinaceus but not for other varieties). The differential band was recovered by gel cutting, then connected to the cloning vector and sent for sequencing. The size of the obtained DNA fragment was 620bp, and its base sequence was shown in SEQIDNo.1. The DNA fragment was Houjie 2 and Big Hericium Share specific DNA segments.

[0032] A primer pair was designed for the specific DNA fragment shared by Houjie 2 and the monkey head. The sequence of the primer pair is:

[0033] Upstream primer:...

Embodiment 2

[0038] (1) Extraction of Hericium erinaceus Genomic DNA

[0039] Take 0.03g of Hericium erinaceus mycelium to be tested, add liquid nitrogen to grind thoroughly, use a new rapid plant genome DNA extraction kit (DP3111, BioTeke, Beijing BioTeke Biotechnology Co., Ltd.) to extract genomic DNA, and obtain improved varieties of Hericium erinaceus Genomic DNA crude extract. The integrity, purity and concentration of the obtained crude genomic DNA were detected by agarose gel electrophoresis and a DNA / RNA UV spectrophotometer (Nanodrop Technologies, USA). DNA samples with OD260 / OD280>1.8 were used for subsequent PCR amplification. DNA extracts were stored in a -20°C refrigerator for later use.

[0040] (2) PCR amplification

[0041]Composition of PCR reaction solution: 2×PowerTaqPCRMasterMix 10μL, 10μM upstream and downstream specific primer pairs 1.5μL each, 20ng / μL template DNA 3μL, ddH 2 04 μL.

[0042] The amplification reaction was carried out on a LifeECO amplification in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus. The primer pair comprises an upstream primer and a downstream primer, wherein the upstream primer is 5'-ATTAGCTTGACGTACAGACAGTCG-3', and the downstream primer is 5'-TGTTGGAGCAAGGGTGATACGA-3'. According to the primer pair, hericium erinaceus houjie No.2 and large hericium erinaceus share one specific molecular marker. The PCR method adopting the primer pair has the advantages that detection time is short, accuracy is high, judgment is easy and repeatability is high when used for identifying hericium erinaceus houjie No.2 or large hericium erinaceus, and early-stage identification can be achieved quickly.

Description

technical field [0001] The invention relates to the technical field of molecular identification, in particular to a pair of primers and a method for identifying Hericium erinaceus No. 2 or Hericium erinaceus. Background technique [0002] Hericium erinaceus (Hericium erinaceus), also known as Hericium erinaceus, got its name only because of its resemblance to Hericium erinaceus. Monkey mushroom, Hericium erinaceus, Hericium erinaceus, is a famous and precious traditional Chinese dish, the meat is tender, fragrant, delicious. Hericium erinaceus is a rare fungus with both edible and medicinal properties, and its fruiting body and fermented hyphae can be used as medicine. With the improvement of people's living standards, Hericium erinaceus has also entered ordinary families, especially after the "SARS", the nutritional and medicinal value of Hericium erinaceus has become more recognized, the market consumption of Hericium erinaceus has increased sharply, and the broad market ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895C12Q2600/156
Inventor 周祖法陆娜宋吉玲
Owner HANGZHOU ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com